Evaluation of Neuroprotection and Antioxidant
Activities via Drosophila Model System in the Active
Principle Derived from Sida Glutinosa Comm. Ex Cav.
- An Aboriginal Ethno-Medicinal Plant Drug Practiced
in the Folklore Medicinal System

Panduranga Murthy G; Leelaja BC; Ravishankar HG; Dharshan Raj CG; Rajesh Kumar

doi:10.26717/BJSTR.2018.11.002171

Research ArticleOpen Access

Evaluation of Neuroprotection and Antioxidant Activities via Drosophila Model System in the Active Principle Derived from Sida Glutinosa Comm. Ex Cav. - An Aboriginal Ethno-Medicinal Plant Drug Practiced in the Folklore Medicinal System

Volume 11 - Issue 5

**Panduranga Murthy G¹*, Leelaja BC², Ravishankar HG³, Dharshan Raj CG⁴ and Rajesh Kumar⁵**

Author Information Open or Close
- ¹Maharaja Institute of Technology Thandavapura-571 302, Visvesvaraya Technological University, Belagavi, India
- ²DOS in Molecular Biology, University of Mysore, Mysore-570 006, India
- ³Hamsageetha Research Foundation, Tumkur-572 102, India
- ⁴PA College of Engineering, Mangalore – 574 153, Karnataka, India
- ⁵University Department of Chemistry, Ranchi University, Ranchi-834 001, India

Received: November 19, 2018; Published: December 10, 2018

DOI: 10.26717/BJSTR.2018.11.002171

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Abstract

Sida glutinosa Comm. ex Cav.- An aboriginal ethno-medicinal plant drug belongs to family Malvaceae, commonly called as ‘Sticky Fanpetals’. It is a sub-shrub which is available in the forest at shady areas along Ravines. The plant was collected at Biligirirangana Hills (B.R. Hills) of Chamarajanagar district, Karnataka, India and taxonomically identified. The therapeutic information was recorded from traditional medicine men during interaction with tribal healers at B.R. Hills. In the study, methanol extract of Sida glutinosa (G) was preliminarily screened for Physico-chemical and Phyto-chemical properties. Further, the antioxidant activity both in vitro and in vivo was carried-out. The neuroprotective study was explicitly carried out using a model system, Drosophila melanogaster (Oeragon K) strain adult male flies. The result reveals that, the extract G exhibited concentration dependent DPPH scavenging activity. The oxidative stress markers employed to access in vivo antioxidant property of G included lipid peroxidation products malondialdehyde (MDA) and hydroperoxide (HP), reduced glutathione (GSH). The modulatory effect of G on superoxide dismutase (SOD) and catalase (CAT) was also determined. The oxidative stress was induced by using paraquat at 15 mm. The concentration of extract for studies was fixed based on LC50 values. There was a significant annihilation in the levels of MDA and HP in case co exposure of G with Par treated flies’ homogenate. Finally, the level of SOD and CAT was brought to near basal level in the homogenate of flies co exposed with G and Par. In negative geotaxis assay; it was found that, G was able to rescue the flies significantly from deteriorating locomotors dysfunctions. The extract G also exhibited significant antibacterial property against the tested strains.

Abbreviations : MDA: Malondialdehyde; HP: Hydroperoxide; GSH: Glutathione; SOD: Superoxide dismutase; CAT: Catalase; ROS : Reactive Oxygen Species; RNS: Reactive Nitrogen Species; PD: Parkinson’s Disease; SCZ: Schizophrenia; AD: Alzheimer’s Disease; NADPH: Nicotinamide Adenine Dinucleotide Phosphate; DPPH: 1,1-diphenyl-2-picryl-hydrazyl; TBA: thiobarbituric acid; SDS: Sodium lauryl sulphate; OPT: o-phthalaldehyde; TEMED : N,N,N,N Tetramethyl ethylenediamine; EDTA: Ethylenediamine tetra acetic acid; ANOVA: One-Way Analysis Of Variance; MIC: Minimum Inhibitory Concentration