Rapid Identification of Bacterial Species Directly from
Enrichment Broth By MALDI-TOF Mass Spectrometry

Malin Jaworski; Johanna Haiko; Baharak Saeedi

doi:10.26717/BJSTR.2018.10.001897

Research ArticleOpen Access

Rapid Identification of Bacterial Species Directly from Enrichment Broth By MALDI-TOF Mass Spectrometry

Volume 10 - Issue 1

Malin Jaworski, Johanna Haiko and Baharak Saeedi*

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- Division of Clinical Microbiology, Karolinska Institutet, Karolinska University Hospital, Sweden

Received:October 03, 2018; Published: October 16, 2018

DOI: 10.26717/BJSTR.2018.10.001897

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Abstract

Rapid identification of bacterial species causing an infection has shown to have positive impact on the clinical outcome. MALDI-TOF MS is a viable and rapid method for identification of bacterial species not only on agar plates but also directly from positive blood culture bottles and can thus shorten the turnaround time significantly. The aim of this study was to evaluate MALDI-TOF MS on liquid enrichment medium as a possible method for rapid identification of bacterial species by comparing it to routine culturing procedures. The broth was harvested when growth was suspected and analyzed using MALDI-TOF MS. An overall sensitivity of 70.4% was achieved when comparing MALDI-TOF MS and routine procedures. In conclusion, the direct identification from the broth showed a substantial degree of correlation to the routine procedures, where identification was performed from the colonies growing on the agar plates.