Is there any Association between Postmenopausal Osteoporosis and Sclerostin Gene Single Nucleotide Polymorphisms in Turkish Women?

Derya Deveci¹*, Zehra Sema Ozkan², Hüseyin Yuce³

Received: July 11, 2018;   Published: July 18, 2018

DOI: 10.26717/BJSTR.2018.06.001429

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Abstract

Objectives: Evaluation of the probable association between bone mineral density (BMD) and SOST gene single nucleotide polymorphisms (SNPs) in postmenopausal Turkish women.

Methods: This prospective, case- control study was carried out with 204 postmenopausal Turkish women. The population was divided into 2 groups with respect to their lumbar spine T score as follows: 126 osteoporotic women with T score lower than -2.5 SD (OP group) and 78 non-osteoporotic women with T score higher than -1 SD (NOP group). Genotyping of 4 SNPs was performed using polymerase chain reaction-restriction fragment length polymorphism.

Results: There was no considerable difference between groups for parameters of age, menopausal period, weight and height. Interestingly we observed 100% homozygote wild GG genotype for rs17885799 G>A polymorphism and 100% homozygous mutant AA genotype for rs851054 G>A polymorphism among whole study population. The frequency of homozygote mutant TT geneotype of rs865429 C>T polymorphism in NOP and OP groups were 78.2% and 73.8% respectively. The frequency of homozygote wild GG genotype of rs17886183 G>A polymorphism in NOP and OP groups were 96.2% and 94.4% respectively. There was no considerable difference between groups for frequencies of all genotypes and alleles of these 4 SNPs. rs865429 C>T polymorphism showed influence on only femoral neck BMD (OR=0.7, 95% CI: 0.866 – 1.846, p=0.000). And only height showed influence on femoral neck and lumbar spine BMD.

Conclusion: The association between femoral neck BMD and SOST rs865429 C>T polymorphism may open new insights on pharmacogenetic studies of osteoporosis.

Keywords: Postmenopausal Osteoporosis; Sost Gene; Polymorphism; Bone mineral density

Abbevations: BMD: Bone Mineral Density; SNPS: Single Nucleotide Polymorphisms; BMI: Body Mass Index; NOP: Non-Osteoporotic; DEXA: Dual Energy X-Ray Absorptiometry; PCR-RFLP: Performed Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism; MAF: Minor Allele Frequency; SPSS: Statistical Package for Social Sciences

Abstract | Introduction | Materials and Methods | Results | Discussion | Conclusion | Acknowledgement | References |