Assessment of Neuroglial Relationships under Photodynamic Treatment Using Fluorescent Visualization of Giant Axons in Crayfish Ventral Nerve Cord

Evgeny Duz and Mikhail S Kolosov*

Received: May 28, 2018;   Published: June 05, 2018

DOI: 10.26717/BJSTR.2018.05.001161

Full Text PDF

To view the Full Article   Peer-reviewed Article PDF

Abstract

There are lots of publications last time about important role of neuroglial interactions in normal physiology of nervous system. Some of them infer that neuroglial interactions may support survival of neuronal and glial cells. Study the mechanisms of neuroglial interactions needs convenient models which are simple nervous system. They could be easily isolated and studied while retain natural neuroglial relations. The purpose of present work was to estimate glia protective properties of giant axons in simple model – crayfish ventral nerve cord, upon photo oxidative stress. To better visualize the axons shape and reveal the periaxonal glial cells, we were filling of the axons with fluorescent label by means of specially devised microinjector. It enabled us to reveal the glial cells which are in close proximity to the axons. Such glial cells were found to have increased survival in compare to more distant ones from the axons under oxidative stress. The obtained data indicate that the crayfish giant axons have glia protective properties, which molecular mechanisms have to be studied.

Keywords: Neuroglial interaction, Oxidative stress, Glia protection, Piezo-driven microinjection, Neuronal tracing

Abstract| Introduction| Materials and Methods| Results| Conclusion| References|