Invitro Evaluation of the Anti Helminthic Activity of Selected Eritrean Traditional Medicinal Plants

Yohana Habte G Amlak ¹, Yohana Ghirme Weldu¹, Yodit Cheay G Silasie ¹, Ariam Yemane Fisehaye¹, Jeevan Jyoti kaushik², Dawit Eman² and Hagos Andom Tuumzghi*²

Received: July 2, 2018;   Published: July 17, 2018

DOI: 10.26717/BJSTR.2018.06.001425

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Abstract

The aim of the study was to determine the anti helimentic activity of two medicinal plants (Taget esminuta and Moringa Olifera) in Eritrea. Fractionated extracts of the plants were prepared using different solvents and their anthelminthic activity was tested on earthworms (Pheritimaposthuma).The histopathological studies of the worms was conducted to compare the histological damage on the test animals in comparison with the standard drug. Methodology the anthelminthic activity was carried out on freshly collected adult earthworms (Pheretimapostuma). The study was carried as per method of Ajaiyeoba et al. [1]. The earthworms of similar length and width were subjected to different concentrations of each plant extract. At the same time, positive and negative controls were carried out. Time taken for paralysis and death of individual worms were recorded.

Results: The highest yield percentage (22%) was obtained with aqueous ethanol and chloroform solvents from Moringa Olifera raleaves and with aqueous-ethanol in case of T.minuta leaves. Chloroform extract of Tagetes diminuta showed the most remarkable activity in paralyzing and killing the earthworms whereas aqueous-ethanolic extract of Moringao life era leaves was the most potent extract than the other extracts. From the histopathological studies it was observed that the leaf extract of Moringa Olifera as well as that of T.minuta have worm icidal effect. The fruit extract of Moringa Olifera has better damaging effect than that of the leaf extract of this plant.

Conclusion: Fore going observation on the effect of the extracts of two selected plants lead us to reach the conclusion that both the leaf and fruit extract of M.olifera as well as the leaf extract of TMinuta have worm icidal effect. To conclude, in future studies, there is need for thorough phyto chemical, clinical and possible studies on molecular mechanism of action of these plants.

Keywords: In vitro Evaluation; Medicinal Plants; Anti Helimentic; Eritrea; Moringa Olifera; Phyto Chemical; Helminthes; Anaemia; Eosinophilia; Filter Paper; Autoclave Tape

Abstract| Introduction| Methods and Materials Used| Results and Discussion| Conclusion| Acknowledgement| References|