Research ArticleOpen Access

Fast Method for Quantitative Determination of Methylene Blue by Micellar Electrokinetic Chromatography

Volume 3 - Issue 3

Michał J Markuszewski*², Krzesimir Ciura¹, Magdalena Buszewska `Forajta², Julia Jacyna², Marta Kordalewska², Michał Szczypior³, Wojciech Połom³, Joanna Nowakowska¹, Marcin Markuszewski³, Marcin Matuszewski³ and Roman Kaliszan²

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- ¹Department of Physical Chemistry, Medical University of Gdańsk, Poland
- ²Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Poland
- ³Department of Urology, Medical University of Gdańsk, Poland
*Corresponding author: Michał J Markuszewski, Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al Gen J Hallera 107, 80-416 Gdańsk, Polan

Received: March 16, 2018; Published: April 03, 2018

DOI: 10.26717/BJSTR.2018.03.000906

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Abstract

The aim of the study was to develop and optimize a simple, fast and applicable method for the quantitative determination of methylene blue. Different parameters, such as composition of separation buffer, pH value and injection time were investigated in order to obtain the best peak shape and reproducibility within the shortest analysis time. The method developed demonstrated linear response over the range of 20- 100 μg/ml. Micellar electrokinetic chromatographic assay proposed is favorable in terms of the overall analysis time and method simplicity.

Keywords:Methylene Blue; Micellar Electrokinetic Chromatography; Method Development

Abbreviations:MB: Methylene Blue; CE: Capillary Electrophoresis; LC: Liquid Chromatography; MS: Mass Spectrometry; DAD: Diode Array Detection; UV-VIS: Ultraviolet-Visible Spectroscopy; SDS: Sodium Dodecyl Sulfate; BGE: Background Electrolyte; MECK: Micellar Electrokinetic Chromatography; CZE: Capillary Zone Electrophoresis; CPA: Corrected Peak Areas