Corresponding author:Prasanta Kumar Mitra, Professor & Head, Department of Medical Biotechnology, Sikkim Manipal Institute of Medical Sciences, Gangtok, India
Received: July 14, 2017; Published: July 31, 2017
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Effect of season on in vitro anti oxidant activity of Astilberivularis Buch. – Ham. Ex D. Don leaves was studied. Leaves of Astilberivularis Buch. – Ham. Ex D. Don were collected in different seasons and in vitro anti oxidant activity of the leaves was measured by superoxide anion generation with the help of xanthine-xanthine oxidase assay and with linoleic acid peroxidation assay as well as by DPPH photometric assay. Total phenol, flavonoids, ascorbic acid and carotenoid contents in the leaves of different seasons were also estimated. Results showed that in vitro anti oxidant activity of the leaves of Astilberivularis Buch. – Ham. Ex D. Don was found maximum during rainy season (June – July). Anti oxidant compounds in leaves like total phenol, flavonoids, ascorbic acid and carotenoids were also present in maximum amount during rainy season. Anti oxidant activity, therefore, was related with high content of total phenol, flavonoid, ascorbic acid and carotenoids in the leaves. Leaves of Astilberivularis Buch. – Ham. Ex D. Don of rainy season should be used to get maximum anti oxidant activity.