Insights of Rv2921c (Ftsy) Gene of Mycobacterium tuberculosis H37Rv To Prove Its Significance by Computational Approach

Shivangi¹, Md Amjad Beg^1,2 and Laxman S Meena*¹

Received: December 14, 2018;   Published: December 17, 2018

DOI: 10.26717/BJSTR.2018.12.002231

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Abstract

After seeing the epidemic of tuberculosis in our country and across world according to WHO report, we right now present with an emergence of treatment/research against this disease. In this study, we therefore exaggerate some important aspects of ftsY (Rv2921c) gene of Mycobacterium tuberculosis (M tuberculosis) which is a GTP binding and hydrolyzing protein. This gene is 1269bp long and contains four GTP binding motif. The above said protein is involved in Signal Recognition Particle (SRP) pathway. The M tuberculosis SRP pathway comprises of two proteins ffh (Rv2916c), ftsY and a RNA subunit 4.5s RNA. This protein interacts with ffh (Rv2916c) gene and another predicted GTP binding protein known as Rv3362c. The said protein is an important part of protein export system which is an essential process for importing and exporting protein that are synthesized in cytoplasm to plasma membrane and other organelles. Thus, this protein might also be important for pathogenesis. This study enlists the effect of disruption of ftsY gene on its interaction with ffh and secretion system. Hence this study might be an important step in the way of eradication of this disease.

Abbreviations : TB: Tuberculosis; M Tuberculosis: Mycobacterium Tuberculosis; AMs: Alveolar Macrophages; RRTB: Resistance to Rifampicin Tuberculosis; MDR-TB: Multidrug-Resistant TB; SDG: Sustainable Development Goals; XDR-TB: Extremely Drug Resistant TB; GTPases: Guanosine Triphosphatases; G-Proteins: GTP-Binding proteins; SRP: Signal Recognition Particle; PDB: Protein Data Bank; NCBI: National Center for Biotechnology Information; SAPS: SAP Application Performance Standard; I-TASSER: Iterative Threading ASSEmbly Refinement; LOMETS: Local Meta Threading Server; RAMPAGE: Ramachandran Plot Analysis; UniProt-GOA: Gene Ontology Annotation.