Extraction and Analysis of Bioactive Compounds from Dipsacus Fullonum and Galium Verum for Lyme Borreliosis Treatment

Maria Kuhtinskaja and Merike Vaher*

Received: November 22, 2018;   Published: November 29, 2018

DOI: 10.26717/BJSTR.2018.11.002121

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Abstract

Four different methods for the extraction of bioactive compounds from the two medicinal plants were compared. Based on qualitative analysis and comparison of chromatograms, all extracts can be characterized by a high content of polyphenols and iridoids. The identification of major bioactive compounds in both plant extracts were carried out using HPLC-MS analysis. More than fifteen different constituents were identified. The highest amount of iridoid glucosides were observed in Dipsacus samples.

Keywords : Bioactive Compounds, Dipsacus Fullonum, Galium Verum, HPLC-MS, Extraction, Lyme Borreliosis

Abbreviations : HWE - Hot Water Extraction; PHWE - Pressurized Hot Water Extraction