Research ArticleOpen Access

Simple, Rapid and Effective Separation of Nuclear Red Blood Cells from Peripheral Blood of Pregnant Women: A preliminary study

Volume 3 - Issue 3

Masato Kantake*

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- Neonatal Medical Center, Juntendo University Shizuoka Hospital, Japan
*Corresponding author: Masato Kantake, Neonatal Medical Center, Juntendo University Shizuoka Hospital, 1192 Nagaoka, Izunokuni, Shizuoka, 410- 2295, Japan

Received: March 16, 2018; Published: April 03, 2018

DOI: 10.26717/BJSTR.2018.03.000907

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Abstract

The analysis of nuclear red blood cells (NRBCs) in pregnant woman is believed to be a practical, noninvasive prenatal test that is much useful for understanding the prenatal fetal status. Here, we show a simple, rapid and effective method to separate NRBC from maternal peripheral blood with preterm labor. We used MACS Technology which is based on MACS MicroBeads, highly specific antibodies coupled to super paramagnetic 50nm particles, MACS Columns containing a matrix of ferromagnetic spheres, and MACS Separators providing a strong magnetic field. This method could enrich the CD45-CD71+ cells from 0.3% of whole blood cells to 93% of separated cells. When the fetal cell ratio in these separated cells is high in some extent, our method may be useful for further analysis. The yield of NRBCs from 2ml of maternal blood by this method is enough to be used for not only targeted DNA methylavtion analysis but whole exome and whole transcriptome analysis using next generation sequencer which may lead to progress in perinatology.