Anti-Inflammatory Effect of Nano Silver in Chronic Rhinosinusitis Mouse Model

Seung Heon Shin*, Mi Kyung Ye, Mi Hyun Che and Dong Won Lee

Received: October 27, 2018;   Published: November 15, 2018

DOI: 10.26717/BJSTR.2018.11.002050

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Abstract

Objective: Nano silver has antimicrobial and disinfectant properties with considerable cellular toxicity. The aim of this study was to investigate the effect of nano-silver on chronic rhinosinusitis (CRS) using an eosinophilic CRS mouse model.

Methods: The CRS mouse model was established with ovalbumin and Staphylococcus aureus enterotoxin B (SEB). Mice were treated with SEB for 16 weeks and nano-silver was intranasal applied three times a week from weeks 9 through 16. The level of inflammatory cell infiltration and chemical mediators in nasal lavage fluid were determined. Histologic changes were observed using hematoxylin and eosin staining and Periodic acid Schiff (PAS) staining.

Results: Intranasal administration of nano-silver resulted in a significant decrease in inflammatory cell infiltration and interferon-gamma levels in nasal lavage fluid. Nano-silver treated mice displayed significantly decreased inflammatory cell infiltration and PAS-positive cells in nasal mucosa. However, interleukin-4 level and epithelial thickness were not influenced.

Conclusion: Nano-silver effectively reduced Th1 inflammatory response in the eosinophilic CRS mouse model. Although, nano-silver had immunomodulatory effects, for the clinical usage further studies are needed to prove the pharmacokinetic and pharmacodynamic characteristics of nano-silver.

Keywords : Nano Silver; Chronic Rhinosinusitis; Mouse Model; Staphylococcus Aureus Enterotoxin B

Abbreviations : CRS: Chronic Rhinosinusitis; SEB: Staphylococcus Aureus Enterotoxin B; PAS: Periodic Acid Schiff; PBS: Phosphate Buffered Saline; NLF: Nasal Lavage Fluid