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Research ArticleOpen Access

Extra Cellular Lipase Production, Purification and Characterization by Using Bacillus Subtilis in Submerged State Fermentation

Volume 10 - Issue 2

Anitha Muthusamy and Leena Grace Beslin*

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    • Department of Biotechnology, Selvamm Arts &Science College (Autonomous), India
    • *Corresponding author: Leena Grace Beslin, Department of Biotechnology, Selvamm Arts & Science College (Autonomous), Tamilnadu, India

Received: October 03, 2018;   Published: October 17, 2018

DOI: 10.26717/BJSTR.2018.10.001913

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Abstract

Lipases are produced extracellular by the bacterial strain Bacillus subtilis in submerged state fermentation was purified from the cell free culture broth by ammonium sulphate precipitation at 70% saturation followed by purification solvent precipitation by acetone and ethanol. DS- PAGE analysis indicated that the enzymes protein molecular mass was 19.4 kDa. The enzyme activity was maximum growth 72 hours of incubation time, with an activity (0.95U/ml). Lipase activity was maximal at 1% concentration of the substrate. Olive oil produced (32.67) units of lipase was the best induces for lipase production enzyme activity. The optimum PH 8 for the activity of lipase.

Abstract | Introduction| Materials and Methods| Conclusion| References|