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ISSN: 2574 -1241
ISSN: 2574 -1241
Received: October 03, 2018; Published: October 17, 2018
DOI: 10.26717/BJSTR.2018.10.001913
Lipases are produced extracellular by the bacterial strain Bacillus subtilis in submerged state fermentation was purified from the cell free culture broth by ammonium sulphate precipitation at 70% saturation followed by purification solvent precipitation by acetone and ethanol. DS- PAGE analysis indicated that the enzymes protein molecular mass was 19.4 kDa. The enzyme activity was maximum growth 72 hours of incubation time, with an activity (0.95U/ml). Lipase activity was maximal at 1% concentration of the substrate. Olive oil produced (32.67) units of lipase was the best induces for lipase production enzyme activity. The optimum PH 8 for the activity of lipase.
Abstract | Introduction| Materials and Methods| Conclusion| References|
International Association of Landscape Archaeology, Czech Glass Society, Czech Republic
Department of Chemistry, Semenov Institute of Chemical Physics, USSR Academy of Sciences, Moscow, Russia
Pharmaco-kinetics, dynamics and Drug Metabolism, Touro College of Pharmacy, USA
Professor of Nuclear Medicine, Faculty of Medicine and Surgery, University of Milan, Milan, Italy
Associate Professor at Department of Breast and Thyorid Surgey, Chongqing General Hospital, China
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