*Corresponding author:GuoLiu YE and DanLi DU, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China
Received: September 27, 2018; Published: October 03, 2018
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Objective: To study the effect of Fucoxanthin on PI3K / Akt signaling pathway in human cervical cancer Hela cells.
Method: We exposed HeLa cells to different concentrations of Fucoxanthin for 24 hours. The effect of Fucoxanthin on PI3K/Akt signaling pathway related proteins was detected by Western blot. In order to analyze the relationship between nuclear transcription factor activity and Fucoxanthin, the activity of NF-kappa B and AP-1 was determined by luciferase reporter gene assay.
Results: Western blot analysis showed that Fucoxanthin inactivated Akt pathway, inhibited Bcl-2 protein level, induced Bax production and caspase-3 cleavage. The Fucoxanthin may inhibit the growth of HeLa cells by inhibiting the PI3K/Akt signaling pathway. Fucoxanthin dose dependently reduced the activation level of NF- kappa B but did not significantly affect AP-1. This indicates that Fucoxanthin inhibits the activation of NF-kappa B in HeLa cells.
Conclusion: NF-kappa B may be the mediator of Fucoxanthin-induced apoptosis in HeLa cells. As an ideal target for tumor therapy, NF-kappa B can regulate some genes involved in the development and progression of cervical cancer and inhibit cell apoptosis by activating several anti-apoptosis genes.
Keywords: Fucoxanthin; cervical cancer; PI3K/Akt; Hela cells