Effect of Fucoxanthin on PI3K/Akt Signaling Pathway in Human Cervical Cancer HeLa Cells

Guo Liu YE*, Li Jie JIN, Ling Ling WANG and Dan Li DU*

Received: September 27, 2018;   Published: October 03, 2018

DOI: 10.26717/BJSTR.2018.09.001819

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Abstract

Objective: To study the effect of Fucoxanthin on PI3K / Akt signaling pathway in human cervical cancer Hela cells.

Method: We exposed HeLa cells to different concentrations of Fucoxanthin for 24 hours. The effect of Fucoxanthin on PI3K/Akt signaling pathway related proteins was detected by Western blot. In order to analyze the relationship between nuclear transcription factor activity and Fucoxanthin, the activity of NF-kappa B and AP-1 was determined by luciferase reporter gene assay.

Results: Western blot analysis showed that Fucoxanthin inactivated Akt pathway, inhibited Bcl-2 protein level, induced Bax production and caspase-3 cleavage. The Fucoxanthin may inhibit the growth of HeLa cells by inhibiting the PI3K/Akt signaling pathway. Fucoxanthin dose dependently reduced the activation level of NF- kappa B but did not significantly affect AP-1. This indicates that Fucoxanthin inhibits the activation of NF-kappa B in HeLa cells.

Conclusion: NF-kappa B may be the mediator of Fucoxanthin-induced apoptosis in HeLa cells. As an ideal target for tumor therapy, NF-kappa B can regulate some genes involved in the development and progression of cervical cancer and inhibit cell apoptosis by activating several anti-apoptosis genes.

Keywords: Fucoxanthin; cervical cancer; PI3K/Akt; Hela cells

Introduction | Materials and Methods | Results| Discussion| Conclusion| Acknowledgment| References|