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Research ArticleOpen Access

The Origin of CD45-CD71+ Cells Enriched by MACS Technology

Volume 8 - Issue 3

Masato Kantake*

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    • Neonatal Medical Center, Juntendo University Shizuoka Hospital, Japan

    *Corresponding author: Masato Kantake, Neonatal Medical Center, Juntendo University Shizuoka Hospital, 1192 Nagaoka, Izunokuni, Shizuoka, 410-2295, Japan

Received: August 16, 2018;   Published: August 27, 2018

DOI: 10.26717/BJSTR.2018.08.001648

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Abstract

Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. In our previous study, we have enriched CD45-CD71+ cells from maternal blood. To confirm the potential usefulness of our method, we analyzed the origin of the enriched CD45-CD71+ cells using a real-time polymerase chain reaction (PCR) system.

The SRY (sex-determining region Y) gene was detected only in cells extracted from the blood of the pregnant woman with the male fetus. The relative dose of the SRY gene to the TFRC (transferrin receptor) gene was 0.001, indicating that 99.9% of the separated cells were from the mother and only 0.1% were of fetal origin.

Our method using MACS technology was insufficient to analyze the fetal genome, methylome, or transcriptome, as some additional enrichment is needed, such as single cell technology

Abstract | Background | Method | Results | Discussion | Conflict of Interest | Acknowledgement | References |