Research ArticleOpen Access

The Interaction of Bovine Serum Albumin with Trimeric (Methyldodecylbis [2- (Dimethyldodecylammonio) Ethyl] Ammonium Tribromide and Make A Comparison with Corresponding Gemini and Monomeric Surfactants

Volume 7 - Issue 4

**Yan Tu¹, Yazhuo Shang¹*, Honglai Liu¹ and Fei Gao²**

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- ¹School of Chemistry & Molecular Engineering, East China University of Science and Technology, China
- ²The 2nd Refinery of Fushun Petrochemical Company of Petro china, Fushun, China
*Corresponding author: Yazhuo Shang, School of Chemistry & Molecular Engineering, East China University of Science and Technology, China

Received: August 01, 2018; Published: August 08, 2018

DOI: 10.26717/BJSTR.2018.07.001542

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Abstract

The mixed systems of bovine serum albumin (BSA) and surfactants were widely used in biological, foods, pharmaceutical and cosmetics. In this work, trimeric (methyldodecylbis [2-(dimethyldodecylammonio) ethyl] ammonium tribromide surfactant (C₁₂-C₂-C₁₂-C₂-C₁₂·3Br-) and Gemini surfactant 1, 2-ethane bis (dimethyldodecylammonium bromide) (C₁₂-C₂-C₁₂·2Br-) were synthesized. And the interaction of trimeric surfactant (C₁₂-C₂-C₁₂-C₂-C₁₂·3Br-), Gemini surfactant (C₁₂-C2-C₁₂·2Br-) and Monomeric Surfactant (Dodecyl trimethyl ammonium bromide: DTAB) with BSA were explored systematically by surface tension, fluorescence methods dynamic light scattering (DLS). The results suggested that the trimeric surfactants have the greatest effect on BSA unfolding comparing with the corresponding Gemini and monomeric surfactants. Furthermore, C₁₂-C₂-C₁₂·2Br- also have much stronger binding ability with BSA to induce the denaturation of BSA at lower concentration of C12-C2-C12·2Br- Compared with DTAB. Those results will attribute to understand the physicochemical effect of surfactant architecture on its interaction with biomacromolecules and broaden their potential applications in medicine.

Keywords: Bovine Serum Albumin; Trimeric Surfactant; Gemini surfactant; Interaction

Abbreviations: DTAB: Dodecyl Trimethyl Ammonium Bromide; DLS: Dynamic Light Scattering; CMC: Critical Micelle Concentration; CAC: Critical Aggregation Concentration; BSA: bovine serum albumin