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Research ArticleOpen Access

Lipopolysaccharide-treated Human Monocytes Regulate Gene Expressions After Interactions with Human Adipocytes

Volume 7 - Issue 4

Teruko Honda*1 and Hiroyuki Inagawa2,3

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    • 1Department of Medical Technology, School of Life and Environmental Science, Azabu University, Japan
    • 2Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Japan
    • 3Control of Innate Immunity Technology Research Association, Department of Research and Development, Japan

    *Corresponding author: Teruko Honda, Department of Medical Technology, School of Life and Environmental Science, Azabu University, Kanagawa, Japan

Received: July 24, 2018;   Published: August 02, 2018

DOI: 10.26717/BJSTR.2018.07.001522

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Introduction: Monocytes infiltrate tissues and differentiate into tissue-specific macrophages by interaction with other cells in tissues. Macrophages in the arterial wall uptake of oxidized LDL and form foam cells and induce inflammatory changes in tissues by secreting inflammatory cytokines. Chronic inflammation is believed to be involved in the development of cancer and lifestyle-related diseases. Whereas, in human monocytes, the mRNA expression of inflammatory factors increases by interactions with cancer cells, however, this increase can be suppressed by pretreatment with low-dose LPS. In the present study, we investigated changes in the gene expression of some key cytokines, inflammatory factors [IL-1 and adiponectin] and a chemotactic factor [MCP-1], after interactions between human adipocytes and LPS-pretreated human monocytes.

Materials and Methods: The human monocyte cell line THP-1 was treated with LPS and subsequently co-cultured with human adipocytes using an insert co-culture system. The gene expressions of inflammatory factors and chemotactic factor were analyzed using quantitative real-time PCR and DNA microarray.

Results: The increased mRNA expression of IL-1β in human adipocytes after co-culture was suppressed by interaction with LPS-pretreated THP-1 cells. The decreased mRNA expression of adiponectin in human adipocytes after co-culture was increased by interaction with LPS-pretreated THP-1 cells. In addition, the increased mRNA expression of MCP-1 in THP-1 cells after interaction with human adipocytes was suppressed by LPS-pretreatment.

Conclusion: LPS-pretreated human monocytes may have anti-inflammatory effect in adipose tissues. LPS-treated human monocytes may be beneficial for the prevention of diseases caused by chronic inflammation.

Keywords: Monocyte, Adipocyte, Co-Culture, Lipopolysaccharide, Inflammatory Factor

Abbreviations: LDL: low density lipoprotein; LPS: lipopolysaccharide; IL: interleukin; MCP-1: Monocyte Chemotactic Protein-1; PCR: Polymerase Chain Reaction; VEGF: Vascular Endothelial Growth Factor; TNF: Tumor Necrosis Factor

Abstract | Introduction | Materials and Methods | Results | Discussion | References |