+1 (502) 904-2126   One Westbrook Corporate Center, Suite 300, Westchester, IL 60154, USA   Site Map
ISSN: 2574 -1241

Impact Factor : 0.548

  Submit Manuscript

Research ArticleOpen Access

Development and Optimization of Immunoenzyme Technique for the Localization of Absicic Acid in Cucumber

Volume 7 - Issue 2

Yan Mei Wu, Dan Yan Yang, Ling Li and Bo Hu*

  • Author Information Open or Close
    • Guangdong Provincial Key Lab of Biotechnology for Plant Development, South China Normal University, China

    *Corresponding author: Bo Hu, College of Life Sciences, South China Normal University, Guangzhou 510631, China

Received: July 04, 2018;   Published: July 27, 2018

DOI: 10.26717/BJSTR.2018.07.001485

Full Text PDF

To view the Full Article   Peer-reviewed Article PDF


An immunoenzymatic method for in situ detection or localization of abscisic acid (ABA) in cucumber leaves were developed and optimized. Samples were sliced by frozen embedding and fixed in 2% EDC(1-(3-Dimethylaminutesopropyl)-3-ethylcarbodiimide hydrochloride) stationary liquid (3% paraformaldehyde, 0.5% glutaraldehyde, 10mmol/LPBS (phosphate buffer saline, pH 7.0) and in 20% sucrose for 1h. The samples were then treated in 0.1% trypsin for 5 minutes at 37 ℃. Sample slices made by this method kept high integrity, the vein cells arranged in order, peripheral cell wall was undamaged and the tissue keep the most strong staining.

Keywords: Bio-acoustic deterrents, sonic weapons, carcinogenesis, loss of hearing, balance, brain injuries, nausea, headaches, ear-ringing, State Department, the U.S. consulate in Guangzhou, China, Havana-Case, Cuba, Secretary of State Mike Pompeo

Abstract | Introduction | Materials and methods | Results and Analysis | Discussion | Conclusion | Acknowledgement | References |