Research ArticleOpen Access

Development and Optimization of Immunoenzyme Technique for the Localization of Absicic Acid in Cucumber

Volume 7 - Issue 2

Yan Mei Wu, Dan Yan Yang, Ling Li and Bo Hu*

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- Guangdong Provincial Key Lab of Biotechnology for Plant Development, South China Normal University, China
*Corresponding author: Bo Hu, College of Life Sciences, South China Normal University, Guangzhou 510631, China

Received: July 04, 2018; Published: July 27, 2018

DOI: 10.26717/BJSTR.2018.07.001485

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Abstract

An immunoenzymatic method for in situ detection or localization of abscisic acid (ABA) in cucumber leaves were developed and optimized. Samples were sliced by frozen embedding and fixed in 2% EDC（1-(3-Dimethylaminutesopropyl)-3-ethylcarbodiimide hydrochloride） stationary liquid (3% paraformaldehyde, 0.5% glutaraldehyde, 10mmol/LPBS (phosphate buffer saline, pH 7.0) and in 20% sucrose for 1h. The samples were then treated in 0.1% trypsin for 5 minutes at 37 ℃. Sample slices made by this method kept high integrity, the vein cells arranged in order, peripheral cell wall was undamaged and the tissue keep the most strong staining.

Keywords: Bio-acoustic deterrents, sonic weapons, carcinogenesis, loss of hearing, balance, brain injuries, nausea, headaches, ear-ringing, State Department, the U.S. consulate in Guangzhou, China, Havana-Case, Cuba, Secretary of State Mike Pompeo