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Research ArticleOpen Access

Hyaline Cartilage Regeneration on Osteochondral Defects by Intraarticular Injection of Human Peripheral Blood CD34+ Stem Cells, Hyaluronic Acid and Growth Factor In a Rat Model

Volume 7 - Issue 1

Basuki Supartono1, Errol Hutagalung2, Ismail2, Arief Boediono3, Toshiro Shirakawa4, Samsuridjal Djauzi5, Ahmad Aulia Yusuf6, Nuryati C. Siregar7, Jacub Pandelaki8, Adang Bachtiar9 and Katsumi Shigemura10

  • Author Information Open or Close
    • 1Medical Faculty of UPN Veteran Jakarta, Indonesia
    • 2Department of Orthopaedic and Traumatology, Medical Faculty of University of Indonesia, Indonesia
    • 3Department of Anatomy, Physiology and Pharmacology, Bogor Agricultural Institute, Indonesia
    • 4Department of Advanced Medical Science, Kobe University Graduate School of Science, Technology and Innovation, Japan
    • 5Department of Internal Medicine, Medical Faculty of University of Indonesia, Indonesia
    • 6Histology Department, Medical Faculty of University of Indonesia, Indonesia
    • 7Department of Pathology Anatomy, Medical Faculty of University of Indonesia, Indonesia
    • 8Department of Radiology, Medical Faculty of University of Indonesia, Indonesia
    • 9Faculty of Public Health, University of Indonesia, Indonesia
    • 10Department of Urology and Divison of Infectious Disease Control Kobe University, Japan

    *Corresponding author: Basuki Supartono, Centre for Study of Cell and Tissue Engineering, University of Pembangunan Nasional Veteran, Jakarta

Received: July 11, 2018;;   Published: July 18, 2018

DOI: 10.26717/BJSTR.2018.07.001436

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Abstract

The knee joint cartilage often suffers from defect and it causes serious health problem. CD34+ stem cells cells have been studied to heal bone fracture however the cells has never been studied to repair cartilage. This study also focuses on the use of scaffold and growth factor affecting the healing of cartilage defect using CD34+ cells. This is the first study reporting hyaline cartilage regeneration on osteochondral defect with intra articular injection of human peripheral blood CD34+ (HPB CD34+) cellated on the trochlear region of Sprague Dawley (SD) rats. A total of 30 male SD rats were randomly divided into 3 groups; the control group received PBS, experimental group 1 received HPB CD34+ cells, and experimental group 2 received HPB CD34+cells, hyaluronic acid, and growth factors (TGF-ß1, IGF-1, FGF, fibronectin). Laboratory, radiology, macroscopic and microscopic evaluations were done on week 4th and 8th. At week 4th and 8th, both experimental groups showed the defects fully filled with hyaline cartilage but not in control group. In conclusion, human peripheral blood CD34+ stem cells can generates hyaline cartilage of osteochondral defects in a rat model.

Keywords: Stem Cell; CD34 + Cell; Hyaline Cartilage Regeneration; Osteochondral Defect; Knee Joint; Scaffold; Growth Factors

Keywords: SD: Sprague Dawley; MSCs: Mesenchymal Stem Cells; TGF: Transforming Growth Factor; IGF: Insulin-Like Growth Factor; FGF: Fibroblast Growth Factor; ACUC: Animal Care and Use Committee; HE: Hematoxylin and Eosin; DAB: Diamino Benzidine

Abstract | Introduction | Material & Methods | Radiological and Macroscopic Evaluation of Knee Joint | Discussion | Conclusion | Acknowledgement | References |