*Corresponding author:Basuki Supartono, Centre for Study of Cell and Tissue Engineering, University of Pembangunan Nasional Veteran, Jakarta
Received: July 11, 2018;; Published: July 18, 2018
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The knee joint cartilage often suffers from defect and it causes serious health problem. CD34+ stem cells cells have been studied to heal bone fracture however the cells has never been studied to repair cartilage. This study also focuses on the use of scaffold and growth factor affecting the healing of cartilage defect using CD34+ cells. This is the first study reporting hyaline cartilage regeneration on osteochondral defect with intra articular injection of human peripheral blood CD34+ (HPB CD34+) cellated on the trochlear region of Sprague Dawley (SD) rats. A total of 30 male SD rats were randomly divided into 3 groups; the control group received PBS, experimental group 1 received HPB CD34+ cells, and experimental group 2 received HPB CD34+cells, hyaluronic acid, and growth factors (TGF-ß1, IGF-1, FGF, fibronectin). Laboratory, radiology, macroscopic and microscopic evaluations were done on week 4th and 8th. At week 4th and 8th, both experimental groups showed the defects fully filled with hyaline cartilage but not in control group. In conclusion, human peripheral blood CD34+ stem cells can generates hyaline cartilage of osteochondral defects in a rat model.
Keywords: Stem Cell; CD34 + Cell; Hyaline Cartilage Regeneration; Osteochondral Defect; Knee Joint; Scaffold; Growth Factors
Keywords: SD: Sprague Dawley; MSCs: Mesenchymal Stem Cells; TGF: Transforming Growth Factor; IGF: Insulin-Like Growth Factor; FGF: Fibroblast Growth Factor; ACUC: Animal Care and Use Committee; HE: Hematoxylin and Eosin; DAB: Diamino Benzidine