Research ArticleOpen Access

Hyaline Cartilage Regeneration on Osteochondral Defects by Intraarticular Injection of Human Peripheral Blood CD34+ Stem Cells, Hyaluronic Acid and Growth Factor In a Rat Model

Volume 7 - Issue 1

Basuki Supartono¹, Errol Hutagalung², Ismail², Arief Boediono³, Toshiro Shirakawa⁴, Samsuridjal Djauzi⁵, Ahmad Aulia Yusuf⁶, Nuryati C. Siregar⁷, Jacub Pandelaki⁸, Adang Bachtiar⁹ and Katsumi Shigemura¹⁰

Author Information Open or Close
- ¹Medical Faculty of UPN Veteran Jakarta, Indonesia
- ²Department of Orthopaedic and Traumatology, Medical Faculty of University of Indonesia, Indonesia
- ³Department of Anatomy, Physiology and Pharmacology, Bogor Agricultural Institute, Indonesia
- ⁴Department of Advanced Medical Science, Kobe University Graduate School of Science, Technology and Innovation, Japan
- ⁵Department of Internal Medicine, Medical Faculty of University of Indonesia, Indonesia
- ⁶Histology Department, Medical Faculty of University of Indonesia, Indonesia
- ⁷Department of Pathology Anatomy, Medical Faculty of University of Indonesia, Indonesia
- ⁸Department of Radiology, Medical Faculty of University of Indonesia, Indonesia
- ⁹Faculty of Public Health, University of Indonesia, Indonesia
- ¹⁰Department of Urology and Divison of Infectious Disease Control Kobe University, Japan
*Corresponding author: Basuki Supartono, Centre for Study of Cell and Tissue Engineering, University of Pembangunan Nasional Veteran, Jakarta

Received: July 11, 2018;; Published: July 18, 2018

DOI: 10.26717/BJSTR.2018.07.001436

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Abstract

The knee joint cartilage often suffers from defect and it causes serious health problem. CD34+ stem cells cells have been studied to heal bone fracture however the cells has never been studied to repair cartilage. This study also focuses on the use of scaffold and growth factor affecting the healing of cartilage defect using CD34+ cells. This is the first study reporting hyaline cartilage regeneration on osteochondral defect with intra articular injection of human peripheral blood CD34+ (HPB CD34+) cellated on the trochlear region of Sprague Dawley (SD) rats. A total of 30 male SD rats were randomly divided into 3 groups; the control group received PBS, experimental group 1 received HPB CD34+ cells, and experimental group 2 received HPB CD34+cells, hyaluronic acid, and growth factors (TGF-ß1, IGF-1, FGF, fibronectin). Laboratory, radiology, macroscopic and microscopic evaluations were done on week 4th and 8th. At week 4th and 8th, both experimental groups showed the defects fully filled with hyaline cartilage but not in control group. In conclusion, human peripheral blood CD34+ stem cells can generates hyaline cartilage of osteochondral defects in a rat model.

Keywords: Stem Cell; CD34 + Cell; Hyaline Cartilage Regeneration; Osteochondral Defect; Knee Joint; Scaffold; Growth Factors

Keywords: SD: Sprague Dawley; MSCs: Mesenchymal Stem Cells; TGF: Transforming Growth Factor; IGF: Insulin-Like Growth Factor; FGF: Fibroblast Growth Factor; ACUC: Animal Care and Use Committee; HE: Hematoxylin and Eosin; DAB: Diamino Benzidine