*Corresponding author:Benoist Chibaudel, Department of Medical Oncology, Franco British Institute, France
Received: June 25, 2018; Published: July 13, 2018
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Background : The development of liquid biopsies could help clinicians to shorten treatment decision and allow longitudinal molecular monitoring. We report here our experience of using the Biocartis IdyllaTM test for patients with advanced colorectal cancer in daily clinical practice.
Patients and Methods: This program prospectively enrolled patients with advanced colorectalcancer at the Department of Medical Oncology of the Franco-British Institute, Levallois-Perret,France.Theprimary objective was to evaluate the feasibilty of the IdyllaTM testing in daily clinical practice.
Results: From November 17, 2017 to June 12,2018, 50 patients were tested. The median time to circulating tumor (ct) extended mutational status was 4.8 hours (95% CI:4.7-5.1). Among 37 (74.0%) patients with paired plasma and tumor samples available, the overall agreement rate was 73.0%. In patients with previously untreated metastatic liver disease (n=9;18%), the overall agreement rate between paired plasma and tumor samples was 100.0%, and the weighted K coefficient was 1.00 (95% CI: 1.00-1.00).
Conclusion : Extended RAS mutational status analysis in circulating tumor DNA from patients with advanced colorectal cancer is feasible and greatly helpful for daily clinical practice. We recommend restricting ctDNA testing with IdyllaTM to patients with previously untreated liver metastatic disease.
Keywords: Colorectal Cancer; Liquid Biopsy; KRAS; NRAS; BRAF
Abbreviations: EGF: Epidermal Growth Factor; MoAb: Monoclonal Antibodies; KRAS: Kirsten Rat Sarcoma Viral Oncogene Homolog; ECD: Extra Cellular Domain; ctDNA: Circulating Tumor DNA; CT: Circulating Tumor