Optimization Strategies for Improving the Resolution of Alkaline Proteins Using 2D-Gel Electrophoresis

Lei Zhang¹, Lihong Weng² and Robert J Hickey*³

Received: May 25, 2018;   Published: May 29, 2018

DOI: 10.26717/BJSTR.2018.05.001131

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Abstract

The resolution of alkaline proteins by 2D-polyacrylamide gel electrophoresis (2D-PAGE) is often accompanied by protein streak artifacts. To overcome this type of artifact formed during traditional 2D-PAGE, and to further improve the resolution of alkaline proteins, we developed a DeStreak ™ protocol that essentially eliminates the streaking artifact through the use of hydroethyldisulfide (HED) throughout the entire IEF run. We found that HED improves the separation and resolution of individual basic proteins when using a narrow range (pH 7-10) IPG strip format.

Keywords: 2D-polyacrylamide Gel Electrophoresis; Isoelectric Focusing; De-streaking

Abbreviations: HED: Hydroxyethyl Disulfide; 2-VP: 2-Vinyl Pyridine

Abstract| Introduction| Materials and Methods| Results and Discussion| Acknowledgement| References|