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Research ArticleOpen Access

IGF-1 Gene P1 Promoter Polymorphisms Influence Predisposition to Epithelial Invasive Ovarian Cancer

Volume 4 - Issue 3

Wojciech Kwasniewski1, Anna GozdzickaJozefiak2, Grzegorz Polak1, Witold Nowak3, Anna Kwasniewska4, Jan Kotarski1 and Maria WolunCholewa*5

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    • 1Department of Gynecological Oncology and Gynecology, Medical University of Lublin,Poland
    • 2Department of Molecular Virology, Adam Mickiewicz University, Poland
    • 3Molecular Biology Techniques Laboratory, Adam Mickiewicz University, Poland
    • 4Department of Obstetrics and Gynecology, Medical University of Lublin,Poland
    • 5Department of Cell Biology, Poznan University of Medical Sciences, Poland

    *Corresponding author: Maria Wolun Cholewa, Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznan, Poland

Received: April 23, 2018;   Published: May 09,2018

DOI: 10.26717/BJSTR.2018.04.001051

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Abstract

Results of recent studies showed that the insulin growth factor (IGF) pathway might be implicated in the development of epithelial ovarian cancer (EOC). Moreover, it was demonstrated that EOC cells with high microsatellite instability (MSI-H; non19/non19) accumulate mutations in the IGF-1 gene. The aim of our study was to analyses the (CA)n repeats polymorphism in the P1 promoter region of the IGF-1 gene in EOC and healthy volunteers.For identification of (CA)n repeats PCR and DNA sequencing were utilised. Serum blood concentrations of IGF-1, IGFBP-1, IGFBP-3 were determined with ELISA. IGF-1 expression was assessed by immunohistochemical analysis. According to the obtained results biological samples were classified as microsatellite instability-high (MSI-H), MSI low (MSI-L) and microsatellite stable (MSS).

The results of the study showed that the most frequent genotype in the control group was MSS and in the EOC group - MSI-H. The IGF-1 level was positively correlated with IGFBP-3 in the MSS and MSI-H genotypes, only. The number of IGF-1-immunopositive cells did not differ between histopathological tissue types. Taking into consideration the genetic instability, the number of IGF-1-immunopositive cells in control group was significantly higher in MSS than in MSI-L and MSI-H genotypes. Between MSI-L and MSI-H genotypes, there was no difference in the number of IGF-1-immunopositive cells. In cancer cells, the number of IGF-1-immunopositive cells did not differ significantly depending on the type of instability observed. Regarding the histopathological diagnosis, low, as well as high instability profiles, were characterized by significantly higher number of IGF-1-immunopositive cells in cancer group. No significant differences were found in MSS profile in the number of IGF-1-immunopositive cells between the control and cancer groups.Present study suggests that IGF-1 (CA)19 gene polymorphisms are associated with occurrence EOC in Caucasian females.

Keywords: CA (19)n P1IGF-I Repeats; IGF-1; IGFBP-1; IGFBP-3; Epithelial Invasive Ovarian Cancer

Abbreviation: EOC: Epithelial Invasive Ovarian Cancer; CA: Cytosine Adenosine; FIGO: International Federation of Gynecology and Obstetrics; ELISA: Enzyme-Linked Immunosorbent Assay; t-SNPs: Tag Single- Nucleotide Polymorphisms

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