Research ArticleOpen Access

Occurrence and Distribution of Chilli Leaf Curl Complex Disease in West Bengal

Volume 3 - Issue 4

**Uday Bikash Oraon* and Jayanta Tarafdar**

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- Department of Plant pathology, BCKV, India
*Corresponding author: Uday Bikash Oraon, Department of Plant pathology, BCKV, Mohanpur, Nadia, West Bengal, India, 741252

Received:March 30, 2018; Published: April 11, 2018

DOI: 10.26717/BJSTR.2018.03.000948

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Abstract

Chilli (Capsicum annum L) is popularly known as ‘wonder spice’ and is an important cash crop and remunerative vegetable crop of India and is grown both for home market and export. Chilli leaf curl complex disease is one of the major limiting factors in chilli production & the reasons behind this complex disease are whitefly (Bemisia tabaci) transmitted begomoviruses, thrips & mites, causing significant reductions in yield and quality of chilli. In our present investigation attempt was taken to study the incidence and distribution of the Chilli Leaf Curl Complex Disease predominantly occurring & infecting chilli crops in different locations of West Bengal & detection of begomoviruses through PCR based method using one pair of degenerate primer SPG1/SPG2. The survey was carried out in 18 villages and total 54 fields in six districts randomly selected in West Bengal during July- August, 2015 when the crop was at 2-3 month old. The highest leaf curl incidence was noticed in Cooch Behar district (30-90%) followed by Burdwan (20-85%) and North 24 parganas (45-75%) in 2015. While the lowest incidence was observed at Purulia (18-43%) district. Among six districts Cooch Behar, Jalpaiguri (Madarihat), Burdwan, North 24parganas, Nadia chilli leaf curl samples showed positive reactions in PCR amplification of a target sequence (920 nt long) specific to begomovirus confirmed the presence of begomovirus in the samples tested. Moreover, these studies supply precise information about the frequency and distribution of distinct begomoviruses in chilli in West Bengal.

Keywords: Survey; Chilli leaf curl; Begomovirus; PCR; West Bengal

Abbreviations: CLCV: Chilli Leaf Curl Virus; CTAB: Cetyl Trimethyl Ammonium Bromide; PCR: Polymerase Chain Reaction; NB: North Bengal; SB: South Bengal