*Corresponding author:Sofoklis Stavrou, Obstetrician and Gynecologist, Alexandra General Hospital, First Department of Obstetrics and Gynaecology, National and Kapodistrian University of Athens, Lourou and Vasilissis Sofias Ave, 11528 Athens, Greece
Received: February 19, 2018; Published: March 08, 2018
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MicroRNAs (miRNAs) are small; noncoding RNA molecules characterized as posttranscriptional negative regulators of gene expression and play a critical role in cell proliferation, cell development, cell differentiation, apoptosis, metabolism, and signal transduction. The miR- 196a2T>C and miR-499A>G polymorphisms have been implicated as risk factors for recurrent spontaneous abortions (RSAs). Mature miR- 196a-2 represses the expression of transcription factor HOXB8, a home box protein involved in implantation, and of annexin A1 (ANXA1) related to anti-inflammation inhibiting NF-κB pathway. Mature miR-499 preferentially inhibits the transcription of SOX6 mRNA, a transcription repressor of FGF-3 associated with cell proliferation and cell differentiation of developing fetal tissues.
Blood samples were collected from 135 patients with RSAs and 97 fertile women, from which genomic DNA was extracted, and PCR and Real-time-PCR were performed to identify the miR-196a2T>C and miR-499A>G genotypes. RSA patients exhibited higher frequency of miR- 196a2CC and miR-499AA+GG genotypes, whereas miR-196a2TT+TC and miR-499AG genotypes have been showed higher frequency in the control group, but the frequency distribution is not significantly different between the comparing groups. Based on our study results, the presence of miR-196a2T>C and miR-499A>G polymorphisms is independent and not related to RSAs. The above results are very important, constituting a data base for doctors and researchers in order to know which miRNAs polymorphisms should be ruled out in the investigation of RSAs. Larger study group should be investigated in order to confirm our preliminary results.
Keywords: MicroRNA; Recurrent spontaneous Abortions; Polymorphism
Abbreviations: RSA: Recurrent Spontaneous Abortions; RSM: Recurrent Spontaneous Miscarriage; UTR: 3-Untranslated Region; SNPs : Single Nucleotide Polymorphisms