Protein Content of Dr Alcaraz Y Col Protocol for Obtaining Plasma Growth Factors in 350 Healthy Patients and Comparison with Other Methods Published in Literature

Alcaraz Rubio Jesús*

Received: August 26, 2017;   Published: September 11, 2017

DOI: 10.26717/BJSTR.2017.01.000345

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Abstract

Introduction: The diversity of procedures for obtaining platelet and plasmatic growth factors, the absence of control in most of them and the growing field of clinical application, makes them necessary methods adequately structured, documented, controlled and tested, playable by any author. The present work aims to introduce and test a specific technique for obtaining PRP, with precise characteristics both production and final composition of compound got, in 350 hematological healthy patients, comparing our results with those obtained by other procedures scientifically tested.

Material and Methods: 350 caucasian patients were selected, 175 male and 175 female with age range between 15 and 65, healthy haematologically. The procedure for obtaining the PRP, consisted of a single centrifugation of the blood sample for 30 minutes at 3500 rpm in a angular shaft of 16 tubes centrifuge serie (CEMCON 2) and micropipetting the protein fraction rich in platelet and plasmatic growth factors and cell through open technique under aseptic conditions in horizontal laminar flow hood Grade A at a temperature of 22°C, with the use of leuco-platelet or Buffy-coat layer (PRP rich in leukocytes).

Results: No correlation between the amount of concentrated platelets and the amount of growth factors finally obtained was observed. The protocol set forth concentrated levels of platelets and leukocytes approximately 3 to 5 times higher than baseline levels with a predominance of mononuclear. Levels of growth factors from 7-10 times greater than the patient’s baseline levels, with little variation in them. The growth factor levels were stable in the blood of each patient within 24 h of treatment between 7 and 9 times higher compared to the previous baseline

Conclusion: Compared with other procedures discussed in the literature; This method achieves concentration between 1.5 and 3 times more platelets in the final product, as we can see in figure 5, with a purification of growth factors overall type VEGF and TGF-B clearly superior, visible in figure 6.

Keywords: Platelet Rich Plasma; Growth Factors; Centrifugation; Buffy-Coat

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