Corresponding author:Aneela Zameer Durrani, Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Pakistan
Received: August 08, 2017; Published: August 28, 2017
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Antimicrobial resistance (AMR) is major problem in treatment of uterine infections in dairy animals. To suggest an alternative treatment, avoiding antibiotics, the effect of ozone as therapy against common bacterial uterine infections in dairy females the present study was carried out in 50 animals at dairy farm in district Sarghoda, Punjab province, Pakistan. The animals with history of repeat breeding and abnormal uterine secretions were selected and randomly divided in two groups. Group A received commercially prepared Ozone(Sanofoam®) intrauterine treatment while group B wasgiven gentamicin sulphate(Gentafar 10%®)@30gm in 1000 ml saline water treat intrauterine. Uterine lavage was taken twice, once before applying treatment and second after 8 hours of applying treatment. Samples were cultured for bacteriology to detect E.coli, F. necrophorum, A. pyogenes and St. pyogenes. Bacteria wise percentage of uterine infection was highest for E.coli (88%) followed by 84%, 68% and 60% for A. pyogenes, F. necrophorum, and St. pyogenes respectively. Difference of the colony forming units before and after applying both treatments for each bacterium was calculated. Results were interpreted statistically. Differences among the groups were considered significant at P < 0.05. E. coli, F.necrophorum and St. pyogenes were highly significant as the P value for group differences was less than 0.05. Group differences among St.pyogenes showed no significance as the P>0.05. The results showed that intrauterine treatment with ozone was more responsive (38/50, 76%) as compared to gentamicin sulphate intrauterine treatment (37/50,74%) in cross bred dairy cows with bacterial infections.