Plasma Lipid Profile and Uric Acid in High Fat Fed Female Rats Treated with Oral Contraceptive

DOI: 10.26717/BJSTR.2017.01.000238

Received: July 18, 2017;   Published: July 31, 2017

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Abstract

A high fat diet is widely known in scientific consensus to be associated with abnormal lipid and uric acid metabolisms. The dose-related influence of Combined Oral Contraceptive (COC) in both human and animal models on blood lipid parameters is still controversial. Therefore the aim of the study is to investigate the effect of high fat diet (HFD) and two different doses of a COC on plasma lipid profile and uric acid in female wistar rats. Rats were divided into vehicle treated, high dose COC (HCOC, receiving 7.5μg Levonegestrel/1.5μg of estradiol), low dose COC, (LCOC receiving 1.5μg Levonegestrel/0.3μg of estradiol), High Fat (HF receiving 20%w/w of vegetable oil (turkey brand) supplemented feed), HF+HCOC (receiving both 7.5μg Levonegestrel/1.5μg estradiol and HFD) and HF+LCOC (receiving 1.5μg Levonegestrel/0.3μg of estradiol and HFD). Rats were given distilled water, HCOC, LCOC, HFD, HCOC+HF and LCOC+HF for 8 weeks. When compared with vehicle group, HCOC treatment led to a significant increase (P<0.05) in total cholesterol (TC). LCOC treatment also caused a significant rise (P<0.05) in total cholesterol, triglyceride and uric acid. Rats fed HFD showed a significantly higher (P<0.05) total cholesterol and Total/HDL-C ratio. HCOC+HF administration led to a significant elevation (P<0.05) of total cholesterol and Low density lipoprotein (LDL-C). LCOC+HF administration produced a significant increase in total cholesterol, LDL-C, Triglyceride/HDL-C ratio, Total/HDL-C ratio, LDL-C/HDL-C ratio and uric acid. In conclusion, combined oral contraceptive and high fat diet administrations resulted in an elevated total cholesterol and uric acid. The effect on Triglyceride/HDL-C, Total/HDL-C, LDL-C/HDL-C and uric acid was dependent on dose of the contraceptive, the effect on TC and LDL was not dose- dependent. Administrations of low dose of combined oral contraceptive and high fat diet may produce a greater cardiovascular risk than administration of high dose of combined oral contraceptive and high fat diet.

Abstract| Introduction| Justification of Study| Aim and Objectives| Materials and Methods| Analysis of Plasma Lipid Parameters and Uric Acid| Estimation Of Plasma High and Low Density Lipoprotein Estimation Using Combi Hdl/Ldl| Estimation of Plasma Triglyceride In Plasma| Estimation of Lipid Profile Ratios| Estimation of Plasmauric Acid| Result| Ldl/Hdl Ratio| Triglyceride/Hdl Ratio| Ldl/Hdl Ratio| Discussion and Conclusion| Conclusion| Recommendation| References|