*Corresponding author:Richard W Cole, New York State Department of Health, Wadsworth Center & Department of Biomedical Sciences; School of Public Health; State University of New York at Albany, Albany, New York, USA
Received: June 08, 2017 Published: June 16, 2017
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3D cell culture has great potential in the field of tissue engineering and regenerative medicine. Three- dimensional ECM-like substances such as Matrigel® mimic natural proteins found in the ECM and result in a more accurate reflection of cell growth within the human body. In our lab, we compared HT1080 cells transfected with the histone H2B-green fluorescent protein (GFP) growing directly on glass (2D) to those in Matrigel® on the basis of cell growth, proliferation, division and morphology. During cell counting, the cells were maintained at a near-homeostatic temperature, humidity and CO2 level in a custom stage top environmental chamber  while being imaged on an Olympus (Melville, NY) IX70 inverted fluorescence microscope. Our results indicated that cell growth, division and proliferation were of greater magnitude in Matrigel® than on a glass coverslip (control). For ultrastructure imaging purposes, a culture of MDCK cells (Canis familiaris, kidney, normal) that were transfected with a pEYFP-Tubulin plasmid subcellular localization vector were used.
Widefield epifluorescent analysis indicated that there was no significant difference in cellular ultrastructure on the basis of flat/rounded morphology and microtubule distribution between the cells grown in glass-bottomed Petri dishes and those in Matrigel®. These results are consistent with our hypothesis that cells in Matrigel® would exhibit greater cell growth, proliferation, and division while maintaining normal cell morphology.
Keywords: Imaging; Fluorescence; Tissue-culture