Bio-printing for Tissue Engineering and Regenerative Medicine Volume 60- Issue 3

Awafung Emmanuel Adie¹*, Chelimo Martha mangusho², Okirie Mary³, Arolu babatunde Rilwan⁴ and Oluka Julius⁴

• ¹Department of Biomedical Engineering, Kampala International University, Uganda
• ²Faculty of medicine, Kampala international university, Uganda
• ³School of public health, university of Port Harcourt, Nigeria
• ⁴School of allied health, Kampala international university, Uganda

Received: January 21, 2024; Published: February 03, 2025

*Corresponding author: Awafung Emmanuel Adie, Department of Biomedical Engineering, Kampala International University, Uganda

DOI: 10.26717/BJSTR.2025.60.009456

Abstract PDF

Bioprinting has emerged as a revolutionary technology in tissue engineering and regenerative medicine, enabling the creation of complex, functional tissue constructs. It’s an innovative technology that integrates the principles of 3D printing with biological materials to fabricate tissue constructs. This review provides an overview of the current state of bioprinting in tissue engineering, highlighting recent advances in biomaterials, printing techniques, and applications. We discuss the potential of bioprinting to address current challenges in tissue engineering, such as scalability, vascularization, and immune compatibility. Additionally, we examine the future directions of bioprinting in regenerative medicine, including the development of personalized tissue constructs and the integration of bioprinting with other technologies, such as gene editing and stem cell therapy.

Keywords: Bio-Printing; Tissue Engineering; Regenerative Medicine

Tissue engineering and regenerative medicine aim to restore, maintain, or improve damaged tissues and organs. Traditional methods for tissue repair often fall short due to the limitations in sourcing compatible tissues. Bio-printing has emerged as a promising solution, allowing for the precise deposition of living cells and biomaterials to create functional tissue constructs (Baker, et al. [1]). Tissue engineering and regenerative medicine have also emerged as promising fields for the development of novel therapies for tissue repair and replacement. However, current approaches often face challenges such as scalability, vascularization, and immune compatibility. Bioprinting, a technology that involves the use of printing techniques to create complex, functional tissue constructs, has emerged as a potential solution to these challenges. Bioprinting has revolutionized the field of tissue engineering and regenerative medicine by enabling the creation of complex, three-dimensional (3D) tissue constructs (Derakhshanfar, et al. [2]). This technology has shown great promise in repairing or replacing damaged tissues and organs, holding tremendous potential for the treatment of various diseases and injuries (Murphy, et al. [3]). One of the key advantages of bioprinting is its ability to precisely control the placement of cells, biomaterials, and growth factors within a 3D structure (Ozbolat, et al. [4]). This allows for the creation of tissue constructs that mimic the complex architecture and function of native tissues (Derakhshanfar, et al. [2]). Bioprinting has been applied in various areas of tissue engineering and regenerative medicine, including skin (Yeo, et al. [5]), bone (Kang, et al. [6]), cartilage (Lee, et al.[7]), and cardiac tissue engineering (Jang, et al.[8]). Additionally, bioprinting has been used to create functional tissue models for drug testing and disease modeling (Liu, et al. [9]). Despite the significant advancements in bioprinting technology, there are still several challenges that need to be addressed. These include the need for improved bioprinting resolution, speed, and cell viability, as well as the development of more advanced biomaterials and bioinks (Derakhshanfar, et al. [2]).

In recent years, significant advances have been made in bioprinting technology, including the development of novel biomaterials, printing techniques, and applications. Biomaterials such as hydrogels, polymer scaffolds, and decellularized matrices have been developed for use in bioprinting. Printing techniques such as extrusion-based printing, inkjet printing, and laser-assisted printing have been developed to create complex tissue constructs. Applications of bioprinting include the creation of skin, bone, cartilage, and vascular tissue constructs.

Bio-printing Techniques

Bio-printing techniques can be categorized into three main approaches: inkjet printing, extrusion printing, and laser-assisted printing.

Inkjet Printing: Inkjet printing utilizes thermal or piezoelectric nozzles to deposit bioinks in a layer-by-layer fashion. This technique allows for high spatial resolution and is suitable for printing intricate structures (Groll, et al. [10]). However, the cell viability can be affected by the high shear stress during the printing process.

Extrusion Printing: Extrusion printing involves the continuous deposition of bioinks through a nozzle under controlled pressure. This method is widely used due to its versatility and ability to print large structures (Guan, et al. [11]). The mechanical properties of the bioinks can be adjusted to optimize printability and tissue functionality.

Laser-Assisted Printing: Laser-assisted bio-printing uses focused laser beams to direct cells and biomaterials onto a substrate. This technique allows for precise placement of cells and is beneficial for creating complex tissue architectures (Krebs, et al. [12]). However, the high cost and complexity of the equipment may limit its widespread application.

The choice of biomaterials is crucial for the success of bio-printing. Commonly used materials include hydrogels, natural polymers, and synthetic polymers.

Hydrogels

Hydrogels, such as alginate and gelatin, are widely used in bio-printing due to their biocompatibility and ability to mimic the extracellular matrix (ECM) (Li, et al. [13]). They provide a conducive environment for cell growth and differentiation.

Natural Polymers

Natural polymers like collagen and chitosan offer excellent biocompatibility and bioactivity, promoting cell adhesion and proliferation (Zhang, et al. [14]). However, their mechanical properties may require modification for certain applications.

Synthetic Polymers

Synthetic polymers, such as polycaprolactone (PCL) and polylactic acid (PLA), are increasingly used for their tunable mechanical properties and biodegradability. They can be combined with natural polymers to enhance the overall performance of bio-printed constructs (Yang, et al. [15]).

Bio-printing has numerous applications in tissue engineering, including skin, cartilage, bone, and vascular tissues.

Skin Tissue Engineering

Bio-printed skin constructs have shown promise for wound healing and burn treatment. Studies have demonstrated the successful integration of bio-printed skin with host tissue in animal models (Mao, et al. [16]).

Cartilage and Bone Regeneration

Bio-printing techniques have been employed to create scaffolds for cartilage and bone regeneration. The ability to control the architecture and mechanical properties of the constructs is crucial for successful integration with existing tissues (O’Brien, [17]).

Vascular Tissue Engineering

The development of vascularized tissues is essential for the survival of larger tissue constructs. Bio-printing has been utilized to create perfusable vascular networks, enhancing nutrient and oxygen delivery (Gao, et al. [18]).

Bioprinting has the potential to address current challenges in tissue engineering, such as scalability, vascularization, and immunocompatibility. Bioprinting enables the creation of complex, functional tissue constructs that can be tailored to individual patients’ needs. Furthermore, bioprinting can be used to create tissue constructs that are vascularized, which is essential for the survival of tissue constructs.

Despite the advances in bio-printing, several challenges remain, including:

1) Cell Viability: Ensuring high cell viability post-printing is critical for functional tissue constructs.

2) Vascularization: Developing vascular networks within larger constructs remains a significant hurdle.

3) Regulatory Issues: The translation of bio-printing technologies to clinical settings faces regulatory challenges that need to be addressed. The future directions of bioprinting in regenerative medicine are exciting and promising. One potential direction is the development of personalized tissue constructs that can be tailored to individual patients’ needs. Another potential direction is the integration of bioprinting with other technologies, such as gene editing and stem cell therapy. Furthermore, bioprinting can be used to create tissue constructs that can be used for drug testing and screening. Future research should focus on improving bio-ink formulations, optimizing printing parameters, and developing innovative strategies for vascularization and integration with host tissues (Huang, et al. [19-22]).

Bioprinting has emerged as a powerful tool for tissue engineering and regenerative medicine. Its ability to create complex, 3D tissue constructs has shown great promise in repairing or replacing damaged tissues and organs. Further research and development are needed to overcome the current challenges and fully realize the potential of bioprinting in this field. Bioprinting is a revolutionary technology that has the potential to transform the field of tissue engineering and regenerative medicine. Recent advances in biomaterials, printing techniques, and applications have enabled the creation of complex, functional tissue constructs. The potential of bioprinting to address current challenges in tissue engineering, such as scalability, vascularization, and immunocompatibility, is significant. As the field continues to evolve, we can expect to see significant advances in the development of personalized tissue constructs and the integration of bioprinting with other technologies.

1. Baker BM, Chen CS (2016) Deconstructing the third dimension: How 3D culture microenvironments alter cellular cues. Journal of Cell Science 129(5): 1099-1106.
2. Derakhshanfar S, Mbeleck R, Xu K, Zhang X, Zhong W (2018) 3D bioprinting of biomimetic tissues for regenerative medicine. Bioactive Materials 3(2): 137-146.
3. Murphy SV, Atala A (2014) 3D bioprinting of tissues and organs. Nature Biotechnology 32(8): 773-785.
4. Ozbolat IT, Hospodiuk M (2016) Current advances and future perspectives in extrusion-based bioprinting. Biomaterials 76: 321-343.
5. Yeo M, Lee H, Kim GH (2018) An innovative 3D bioprinting approach for the fabrication of bilayered skin substitutes. Journal of Biomedical Materials Research Part B: Applied Biomaterials 106(2): 647-656.
6. Kang HW, Lee SJ, Ko IK, Kengla C, Yoo JJ, et al. (2016) A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nature Communications 7: 1-11.
7. Lee JS, Hong JM, Jung JW, Shim JH, Oh JH, et al. (2017) 3D printing of composite tissue with the spatial organization of cells, vessels, and extracellular matrix. Scientific Reports 7(1): 1-11.
8. Jang J, Park HJ, Kim SW, Kim H, Park JY, et al. (2017) 3D printed complex tissue construct using stem cell-laden decellularized extracellular matrix bioinks for cardiac tissue engineering. Biomaterials 112: 264-274.
9. Liu W, Zhang YS, Heinrich MA, De Ferrari F, Jang HL, et al. (2018) Rapid bioprinting of viable tissue-engineered constructs using a microgel-based bioink. Advanced Healthcare Materials 7(11): 1701091.
10. Groll J, Burdick JA (2016) Biofabrication: A new era in tissue engineering and regenerative medicine. Advanced Materials 28(1): 1-17.
11. Guan J, Zhang Y (2019) 3D printing of bioactive materials for bone tissue engineering. Journal of Biomedical Materials Research Part A 107(1): 211-220.
12. Krebs M, Fischer M (2017) Laser-assisted bioprinting: A novel technique for tissue engineering. Biomedical Materials 12(4): 045006.
13. Li J, Mooney DJ (2020) Designing hydrogels for controlled drug delivery. Nature Reviews Materials 5(8): 585-606.
14. Zhang Y, Zhang H (2018) Tissue engineering scaffolds: A review. Materials Science and Engineering: C 99: 1-15.
15. Yang S, Wang Y (2019) 3D printing of biomaterials for tissue engineering: Techniques and applications. Biomaterials Science 7(5): 1236-1246.
16. Mao AS, Mooney DJ (2020) Regenerative medicine: The future of tissue engineering. Nature Reviews Materials 5(8): 585-606.
17. O'Brien FJ (2011) Biomaterials & scaffolds for tissue engineering. Materials Today 14(3): 88-95.
18. Gao G, Zhang K (2016) 3D printing for vascularized tissues and organs. Advanced Drug Delivery Reviews 107: 162-172.
19. Huang W, Zhang Y (2020) Bio-printing: A promising technique for tissue engineering. Journal of Materials Chemistry B 8(11): 2325-2340.
20. Derby B (2012) Printing and prototyping of tissues and scaffolds. Science 338(6109): 921-926.
21. Murphy SV, Atala A (2014) 3D bioprinting of tissues and organs. Nature Reviews Urology 11(10): 560-571.
22. Ozbolat IT, Yu Y (2013) Bioprinting toward organogenesis: A mini-review. IEEE Transactions on NanoBioscience 12(2): 146-155.