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Sammani Auwalu1*, Maikaji FS2 and Yusuf Hameedat3
Received: August 02, 2022; Published: August 26, 2022
*Corresponding author: Sammani Auwalu, Ministry of Education Science and Technology, Jigawa State Nigeria
‘Fura da nono’ is a commonly food drink in northern part of Nigeria that is mostly consumed by almost all group of people in the country to include both young, adult and aged ones. The research work was aimed at determining the prevalence of E.coli O157:H7 from ‘fura da nono’. A total of 228 ready-to-take ‘fura da nono’ samples were collected from Samaru, Sabongari and Tudun-wada markets. Proximate composition was carried out to determine the % moisture, ash content, protein content, fat and carbohydrate of the sample. Total viable count was also carried out in order to determine the microbial load. Escherichia coli O157:H7 was isolated using cultural method and confirmed using latex agglutination test. The results obtained revealed that the ‘fura da nono’ samples collected from Samaru market had the highest count of 1.59x108±62.91cfu/ml, followed by that collected from Tudun-wada market had a count of 1.07x108±44.94cfu/ml, while ‘fura da nono’ samples from Sabongari market have the least count of 6.65x107±24.90 cfu/ml. The prevalence of E.coli O157:H7 was found to be 0.88% (2/228). As such, the food drink ‘fura da nono’ used for the study can be contaminated with pathogenic microorganism such as E.coli O157:H7 whose detection in food poses a threat to the health of the consumers. Strict hygienic measures should be adhered to when preparing ‘fura da nono’.
Keywords: Fura Da Nono; E.Coli O157:H7; Zaria
‘Fura da Nono’ (Fermented Milk Cereal Mix) is a highly nutritious food drink which is a two in one product consisting of “fura” made from millet and “nono” a fermented milk product similar to yoghurt. In the market, ‘fura’ is mixed with ‘nono’ in a bowl for consumers. Depending on the consistency, the product is used as food and a refreshing drink to both group of people including the young and elderly Umoh, et al. [1,2]. In Nigeria, the methods of processing and selling cow’s milk and its products such as ‘fura da nono’ exposes these products to the danger of microbial contamination both from spoilage and pathogenic microorganisms. Raw milk or processed milk is a well-known medium that supports the growth of several microorganisms, which results in the contamination of the product as well poses a health threat to the consumers Oliver, et al. . Cattle are considered to be one of the primary sources of E. coli O157:H7 worldwide, as numerous studies have shown that E. coli O157:H7 prevalence is widespread in dairy and beef ani mals, and can be found in, and around cattle in most parts of the world without causing any disease symptoms Muhammed, et al. . Transmission is via the faecal–oral route, and most illness has been through consumption of contaminated raw green vegetables, undercooked meat as well as milk and its products such as ‘fura da nono’ Lim, et al. .
A total number of 228 samples of ready-to-take ‘fura da nono’ samples were collected from three different markets in Zaria town, Kaduna State Nigeria (76 samples from each market). The samples were transported in an ice bag to the Department of Microbiology, Ahmadu Bello University Zaria for the analysis for the analysis. A volume of 25ml of each of the samples was added to 225ml of buffered peptone water to make 1:10 dilution and marked as stock solution. From the stock solution, 1ml was pipetted into a 9ml of buffered peptone water to make the second dilution (10-2). The process was repeated until a dilution of 10-5 was reached. A volume of 0.1ml was pipette onto the surface of plate count agar and spread throughout the plate using a bent glass rod to ensure evenly distribution of the aliquot in duplicate Yusuf, et al. . The procedure was done for all the samples collected.
The proximate composition of each of the samples awas determined using the methods described by AOAC 2000.
Each of the samples was streaked on to the surface of Eosin Methylene Blue agar to detect the presence of a lactose fermentor (Table 1). The colonies that appeared greenish metallic sheen with dark centers were presumptively picked, stored in a nutrient agar slant and considered as strains of E.coli before further confirmed using conventional biochemical test Edward, et al. . The stored organism was subjected to biochemical test such as Indole test, Citrate utilization test, MRVP test MacWilliams . Those isolates that were confirmed to be strains of E.coli based on the mentioned biochemicals were labeled and used for the detection of E.coli O157:H7. The isolates were then subcultured on to the surface of Sorbitol MacConkey agar for the detection of non-sorbitol fermenting organism (Table 2). The isolate that appears colorless were presumptively considered to be strains of E.coli O157:H7. This isolate was further subcultered into nutrient broth for 24hr and subjected to latex agglutination test to confirm the organism (Table 3). The latex agglutination test was carried out based on the manufacturer’s instruction (E.coli O157 antiserum (Oxoid) provided in the kit and the organism was considered positive when agglutination was seen in all the two wells lebelled for O157 and H7 Yusuf, et al. .
Table 3: Frequency of occurrence of E.coli O157:H7 in ‘Fura da Nono’.
Note: χ2: 1.009; P-value: 0.604
The average proximate composition of ‘fura da nono’ as presented in Table 4.1 reavealed the average moisture content of (81.78%), ash content (0.59%), fat (4.97%), crude protein (3.77%) and carbohydrate (8.89%).
The research has shown the average count for samples collected from Samaru market to be 1.59x108±62.91SD (cfu/mL), that of Tudun-Wada to be 1.07x108±44.94 SD (cfu/mL) and the count for Sabongari was found to be 6.65x108±24.90SD (cfu/mL) The highest count was seen in the samples collected from Samaru market.
The result revealed that out of the total number of 228 samples collected from the three sampling areas to include Samaru, Tudun- wada and Sabongari markets, 20 E.coli isolates were obtained and only 2 isolates were confirmed to be strains of E.coli O157:H7 based on latex agglutination test as presented in Plate I. The occurance of E.coli O157:H7 in Samaru market and Sabongari market was found to be 1.13% each, while zero prevalence in Tudun-wada market was observed as no E.coli O157:H7 was isolated from the samples collected in the market.
The proximate composition for a randomly selected sample indicates that the ‘fura da nono’ sample has a moisture content of 81.78%. The moisture content is a measure of water content of the product which accounts for the texture of the food drink. The value obtained in the study might be as a result of water added during the preparation as some of the sellers do add water to the mixture. The value from the study lower than the value of 84.92% obtained by Onoriode, et al.  in Port Harcourt, and higher than the value of 75% obtained by Nneoma  in Abuja. The differences in the value can be attributed to the methods employed in the preparation of the product (‘fura da nono’), season of the year at which the research was carried out. The ash content indicates that the product was rich in minerals which make it a suitable environment for microbial growth. The ash content was found to be 0.59% which was similar to the value obtained by Nneoma  in Abuja, and higher than the value of 0.48% obtained by Onoriode, et al.  in Port Harcourt. The differences in the values may be due to production processes as some Fulani women selling the product due heat the ‘nono’ at high temperature and this may cause the destruction of some essential components of the ‘nono’. The crude lipid value obtained from the study was 4.97%, and this indicates that the ‘fura da nono’ used contains enough fatty acid that can be channeled to metabolic activities through the processes of beta-oxidation. The value was higher than the value of 3.00 obtained by Nneoma  and lower than the value of 3.09 obtained in Port Harcourt by Onoriode, et al. .
The protein content was found to be 3.77%, which indicate that ‘fura da nono’ is rich in nitrogen required for both the growth of human cells as well as the pathogenic organisms. As a result of the location, feeds of the animal and also the season of the year during which the study was carried out, the value was lower than that obtained in Abuja by Nneoma  and higher than that obtained by Onoriode, et al.  in Port Harcourt. The carbohydrate value was found to be 8.89%, and it indicates the suitability of ‘fura da nono’ to be rich in sugars that are used for energy generation. The value obtained was lower than 14% obtained in Abuja by Nneoma  and higher than the value obtained by Onoriode, et al.  of 7.70% in Port Harcourt. The differences in the values may be due to geographical location, production processes, sample size and season of the year as usually the number of the samples used during the research varied. Also the study reveals that ‘Fura da Nono’ sold locally in Zaria has a viable count higher than the standards set by both World Health Organization (WHO) and National Agency for Food and Drugs Administration and Control (NADAC) which suggests a value not exceeding 3x103cfu/ml. The counts range from 1.59x108±62.91SD cfu/ml in Samaru market, 6.65x107±24.90SD cfu/ml in Sabongari market and 1.07x108±44.94SD cfu/ml in Tudun- wada maket. The high counts seen may be due to the unhygienic practices during milking by those selling and handling the product, unclean environment where milking activity is carried out and probably failure to use portable water for cleaning the utensils used during milking and selling of the product.
Also, the environment where the consumers purchase the product and the fact that cattles do pass-bye the side of this areas and during the passage, the cow’s dung might be carried to the area by air inform of aerosols. This shows the role of the environment in the contamination of the local product as suggested by Abid, et al. . The prevalence of E. coli O157:H7 was found to be 0.88% (2/228) at χ2 1.009, P-value0.604 which is not significant. The value obtained was lower than that of 18% reported by Edia-Asuke, et al.  in Zaria, higher than the value of 1.3% also obtained by Ayuba  in Zaria and also higher than that was obtained by Lim, et al.  a prevalence of 0.71% was seen in locally fermented milk (Nono) in Plateau State, Nigeria. The value obtained also was lower than the value obtained by Rubena, et al.  value of 4.5% prevalence from Nasarawa State and also lower than what was obtained by Dahiru, et al. [15,16] from Kano(value of 53%).This value obtained can be attributed to factors such as geographical location, sample size, nature of samples, methods employed as well as the time period during which the research was carried out; the research was carried out during rainy season.