Cardiovascular Disease Prevention: New Nutrigenetics Tests Volume 5 - Issue 4

AV Nagay*, GA Khamidullaeva and RD Kurbanov

• Department of Arterial Hypertension and Molecular Genetics Research, Republican Specialized Scientific-practical Medical Center of Cardiology, Tashkent, Uzbekistan

Received: June 11, 2018;   Published: June 15, 2018

*Corresponding author: Aleksandr Nagay, Department of Arterial Hypertension and Molecular Genetics Research, Republican Specialized Scientificpractical Medical Center of Cardiology 4, Osiyo Str Mirzo-Ulugbek 100052, Tashkent Uzbekistan

DOI: 10.26717/BJSTR.2018.05.001234

Abstract PDF

Keywords: Hypertensive Patient; Residual Risk; Prognostic Model; Nutrigenetics Panel; Nutrigenetics Tests

Nutrigenetics testing undoubtedly is an important part in clinical practice. Each patient has a combination of the genes bound to cardiovascular illnesses. We represent step-by-step the projection of Nutrigenetics tests for cardiologists. We represent the multiplex DNA system with technology of the effective analysis of genes. The technology of the effective genes analysis is the combined method of the sequential analysis, ROC-curve and relative risk of RR (Altman’s theorem) with use of basic mathematical algorithms (Bayes’ theorem) in diagnostics of in vitro. For the first time we applied this technology for identification of genes mutations which enlarge a susceptibility to rising of weight, and also sensitivities to some ions. Thus, using DNA identification, the developed algorithm can recommend an individual diet. The algorithm is capable to transform pathogenic genetic data to the diagnostic tool which in a combination with clinical observation and biometric data can create the clinical decision.

The analysis showed a tendency to a violation of water-salt metabolism in patients with TT- genotype of CYP11B2 gene and DD- genotype of the gene ACE gene via multivariate analysis. This displacement may be observed due to renal dysfunction caused by CYP11B2 and ACE genes regulation, the mechanism of Na+ - exchange through diuresis. The detected connection of 4a/4b-genotype (eNOS gene) and 9+/+9 (B2BKR gene) with the development of endothelial dysfunction in 24% and 8% cases respectively, is most likely due to coupling of the polymorphic marker and site mutation leading to a change of NO production by endothelial cells. The identified association of T/T-genotype of ADRB3 gene and Glu/ Glu-genotype of β2-AR gene with the developing insulin resistance risk in 50% and 8% cases respectively confirmed that these genes mediate the physiologic effects of adrenaline. Genotyping of the SNP was performed by Real-Time polymerase chain reaction and multiplex PCR analysis (DT PRIME Real-Time PCR Systems).

We selected clinical signs for creation of the prognostic table by means of the method of the consecutive diagnostic procedure based on a technique of the sequential analysis offered by A. Wald. For each informative sign gradation of this or that indicator were selected to equal the diagnostic value of each of indicators. Thus, by means of point system the personal diet (KETOGENIC, DASH or balanced diet) for a period of 10 days is prescribed. As it is low - saline diet is a low-calorie diet with small amount of fat and edible salt the value of the “a” sum and the “b” sum are united in one low - a saline diet. These approaches are providing more robust and clinically relevant gene-diet interactions. The final form of the prognostic table is presented in Table 1 and Figure 1 [1-3]. The main characteristics of the offered diets are consolidated in Table 2.

The first time we applied for technology of residual risk on the SNP platform to 3 systems: ENDOTHEL, SNS and RAAS. Depending on the genetic status, this technology allows to correct a food or to apply alternative therapy. The presented method is effective only for the Uzbek population. Results of 5 years of researches (500 Hypertensive patient /400 control group), within scientific grants on studying of nutrigenetics in a clinical cardiology were accepted to basic parameters.

Table 1: Nutrigenetics panel/Cardiovascular risk factors.

Table 2: Characteristics of 3 basic diets.

Figure 1: The phenotype/genotype-specific therapy: new therapeutic concept.

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