*Corresponding author:Oran Kwon, Department of Nutritional Science and Food Management, Ewha Womans University, 52 Ewhayeodaegil, Seodaemun-gu, Seoul 03760, Republic of Korea
Received: October 08, 2018; Published: October 24, 2018
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Background/Objectives: Given that western diet (WD) intake has negative impact on development of non-alcoholic fatty liver disease (NAFLD), the protective effects of Angelica keiskei juice (AK) against hyperlipidemia and hepatic steatosis were investigated in WD-fed C57BL/6J mice.
Materials and Methods: Animals were assigned into four groups (n=8/group) to receive normal diet + vehicle, WD + vehicle, WD + low AK (2 g/kg body weight), and WD + high AK (4 g/kg body weight) for 18 weeks. Biochemical analysis was performed for lipid profile in plasma; histological assessment for steatosis, inflammation, and tissue damage in the liver and intestine; and qPCR analysis for intestinal lipoprotein lipase and hepatic endoplasmic reticulum (ER)/oxidative stress markers.
Results: AK significantly attenuated WD-induced increases in plasma triglyceride and very low density lipoprotein (P=0.001 for both), and hepatic triglyceride (P< 0.001). Particularly, high-dose AK was effective in maintaining intestinal barrier integrity and increasing mRNA expression of hepatic nuclear factor erythroid 2-related factor 2 (P=0.003). It also tended to modify mRNA expressions of intestinal lipoprotein lipase (P=0.004), and hepatic carnitine palmitoyl transferase 1α (P=0.038), endoplasmic reticulum nuclei-1 (P=0.014), and NAD(P)H: quinone oxidoreductase 1 (P=0.047). Conclusions: These findings suggest that daily consumption of AK juice may have potentials to prevent WD-induced NAFLD development through mitigating intestinal barrier damage, intestinal lipid absorption, and hepatic ER oxidative stress.
Keywords: Angelica Keiskei Juice; Western Diet; Hyperlipidemia; Non-Alcoholic Fatty Liver Disease; C57BL/6J Mice
Abbreviations: WD: Western Diet; NAFLD: Non-Alcoholic Fatty Liver Disease; AK: Angelica Keiskei Juice; ER: Endoplasmic Reticulum; TG: Triglyceride; HESI: Heated Electrospray Ionization; NC: Normal Diet, WC: WD Control; EDTA: Ethylene Diamine Tetra-Acetic Acid; IACUC: Institutional Animal Care And Use Committee; TC: Total Cholesterol; HDL: High-Density Lipoprotein Cholesterol; LDL: Low Density Lipoprotein; VLDL: Very Low- Density Lipoprotein; H&E: Haematoxylin and Eosin; UPR: Unfolded Protein Response