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Research ArticleOpen Access

The Minimum Inhibition and Growth Concentrations for Controlling Fungal Infections as well as Ghost Cells Preparation: Aspergillus flavus as a Model

Volume 10 - Issue 2

Nawal Abd El-Baky§1, Mona M Sharaf1, Eman Amer1, Hoda Reda Kholef1, Mohamed Zakaria Hussain2, Raoufa Ahmed Abdel Rahman3 and Amro A Amara*§1<

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    • 1 Protein Research Department,Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technological Applications, Alexandria, Egypt
    • 2Department of Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta
    • 3Pharmaceutical Bio-Products Research Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technological Applications, Alexandria, Egypt
    • §Both authors contributed equally to this study
    • *Corresponding author: Amro Abd Al Fattah Amara, The head of the Protein Research Department, City for Scientific Research and Technological Applications Universities and Research Centre District, New Borg El-Arab, Egypt

Received: September 28, 2018;   Published: October 23, 2018

DOI: 10.26717/BJSTR.2018.10.001926

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There is an increasing interest toward the understanding of the important fungus, Aspergillus flavus, which has a high impact on our health and economy. Controlling such microbe could be achieved by evacuating its cells using simple tool. Recently, the Sponge-Like protocol for bacterial ghosts’ preparation was introduced. Then, this protocol concept was extended to include not only bacterial cells but also yeast cells as well as viruses. In this study, for the first time we applied this protocol to pathogenic fungi as Aspergillus flavus turning it to unviable, dead and evacuated ghost cells. We introduce the idea of using the minimum growth concentration (MGC) and the minimum inhibition concentration (MIC) for controlling Aspergillus flavus and other type of fungi either in our food or those infecting us. Using mixture of compounds proves to have effect on certain microbes upon the use of their minimum effect, which could be a solution for treating many diseases without damaging the cells and tissues as well as our organs. A. flavus which is responsible for causing different superficial infections besides its ability to produce hazardous aflatoxins was used as a model in this study. The future will show an increasing interest in this protocol either for ghost cells preparation or controlling pathogens with minimum side effects of the used destabilizing and killing compounds or drugs. Any chemical compound that could achieve the same goal is invited. We strongly recommend and encourage conducting research in this field using the same or similar tactics and idea.

Abbreviations: Minimum Growth Concentration (MGC); Minimum Inhibition Concentration (MIC); Chronic Cavitary Pulmonary Aspergillosis (CCPA); Allergic Bronchopulmonary Aspergillosis (ABPA); Allergic Fungal Sinusitis (AFS); Phosphate Buffered Saline (PBS); Bovine Serum Albumin (BSA); Transmission Electron Microscope (TEM)

Abstract| Introduction | Materials and Methods| Results and Discussion| Conclusion| References|