Effect of Agaricus Sylvaticus (Schaeffer) Extract in Rats Skin Wound Healing

Gildiney Raimunda da Silva¹, Victor Franklin¹, Joseanne Meira Cambuí¹, Danilo Tenório de Almeida¹, Nilsa Sumie Yamashita Wadt², Vinicius O Cardoso³ and Erna Elisabeth Bach*³

Received:October 07, 2018;   Published: October 16, 2018

DOI: 10.26717/BJSTR.2018.10.001903

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Abstract

Mushrooms are an excellent food option for diets, since they are nourishing and not fattening, mainly due to their nutritional characteristics. They have a high protein content, low lipid values, besides the presence of vitamins (thiamine, riboflavin, niacin), minerals (calcium, iron, phosphorus), beta-glucans and antioxidant compounds. Several people are using it in Brazil as a blood glucose level controller and Anvisa released the Agaricus blazei as a nutraceutical product. In the present project the objective was to evaluate the healing of open cutaneous wounds in rats treated with topical use of Agaricus sylvaticus extract 10% in gel by macroscopic analysis of the cicatricial process. For skin healing of wound in rats, a total of 15 adults male Wistar rats (UNINOVE Vivarium) were used, divided in three groups. Animals in group 1 were treated with 1mL of gel at 10% Agaricus; group 2 with 1mL of distilled aqueous gel and group 3 with fibrinase. Daily applications were performed over the wound of total 4cm2 area in dorsal region of each animal. The wound evaluation was made macroscopically in the time stamps of 0, 7, and 14 days, and the wound evolution, and skin healing retraction measures evaluated by digital planimetry.

Results indicated that extract from Agaricus presented proteins, phenols and beta glucan. As phenols chlorogenic, cafeic, coumaric and benzoic acid were presented and provided antioxidant reaction. The animals treated with Agaricus showed increased healing when compared with control and fibrinase treatment. After 14 days, the wounds in animals treated with Agaricus were healed corresponding to 73.79% of control group, while animals with fibrinase corresponded to 56,58% of control group. This demonstrates that fibrinase was not as efficient as Agaricus and when compared to control group. Thus, since Agaricus has an antioxidant factor and acted as a healing agent, it can be stated that the presence of phenols in the extract was important. It is possible to conclude that the gel with Agaricus is effective at skin healing in rats.

Keywords : Agaricus; Cicatrization; Skin

Abstract | Introduction| Materials and Methods| Results and Discussion| Conclusion| Acknowledgement| References|