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Research ArticleOpen Access

Complete the Manufacturing Procedure of Kit for Determination of Fructose Concentration in Seminal Fluid For Diagnosis Male Infertility

Volume 8 - Issue 1

Nguyen Thi Trang1*, Trieu Tien Sang2, Bui Bich Mai1, Nguyen Hoang3 and Vu Thi Huyen4

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    • 1Department of Biomedical and genetics, Hanoi Medical University, Vietnam
    • 2Department of Biomedical and genetics, Vietnam Military Medical University, Vietnam
    • 3Hanoi - Amsterdam High school for the Gited, Vietnam
    • 4Hanoi Medical University, Vietnam

    *Corresponding author: Nguyen Thi Trang, Department of Biomedical and genetics, Hanoi Medical University, Viet Nam

Received: August 12, 2018;   Published: August 17, 2018

DOI: 10.26717/BJSTR.2018.08.001603

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Introduction: Fructose is formed in the seminal vesicle under the action of testosterone and is secreted along with the vas deferens during each ejaculation, so fructose is considered a biochemistry that faithfully reflects the function of these components. Fructose concentrations in normal semen confirm the role of testosterone and the function of normal seminal vesicles, without the phenomenon of obstruction.

Materials and Methods: The semen samples were obtained from 300 male partners of infertile couples who attended the Department Biomedical and Genetics of the Hanoi medical university and the semen samples were analyzed for the routine seminal parameters. Fructose concentration was assessed using spectrophotometry (improved ROE method).

Results: Optimal solution concentration of TCA is 10%. Composite color composition include 2,5 ml of HCL 30% and 0,25 ml of resorcinol 0,1%; Repeatability: CV% = 1, 407 % <5%; Intermediate precision: CV% = 2.032 % < 5%; Trueness: tex= 0,906 <tt = 2.262. Specificity was 98,9 % and 99,52 % sensitivity. Significant correlation between the two methods with r=0,990, p<0,001; average difference between the two methods is 0,0116 with equivalent is 2,078 % (p=0,035< 0,05).

Conclusion: Complete the manufacturing procedure of kit for determination of fructose concentration in seminal fluid.

Keywords: Phyllanthus orbicularis; Antioxidant; Desmutagen; DFT; AM1 semi-empiric model

Abstract | Introduction | Materials and Methods | Method | Result | Discussion | Conclusion | Acknowledgement | References |