*Corresponding author:Dr. Naresh Kumar Sood, Department of Teaching Veterinary Clinical Complex, Guru Angad Dev Veterinary and Animal Sciences University, College of Veterinary Science, India
Received: July 16, 2018; Published: July 30, 2018
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Objective: To determine the immunolocalization and cellular expression of adenosine triphosphate-binding cassette (ABC) proteins viz. breast cancer resistance protein (BCRP) and multidrug resistance protein 1 (MDR1) in canine mammary tumour (CMT), as well as to correlate their expression with the histological subtypes and grades of CMT with a view towards therapeutic implications.
Methods: Fifty-three cases of malignant CMT were included in the study. The histological sections taken from archived paraffin blocks were subjected to histopathology and imunohistochemistry for sub-typing, grading, immunolocalization and cellular expression of BCRP and MDR1 and their interrelation.
Results: Both BCRP and MDR1 were mainly immulocalized in cellular membranes and to a lesser extent in the cytoplasm of the cancerous epithelial cells and stromal connective tissue. Carcinosarcomas followed by carcinomas and sarcomas revealed decreasing expression of both BCRP and MDR1 proteins. A positive and an inverse correlation between BCRP and MDR1 expression, respectively was observed with the increasing grade of CMT.
Conclusion: The study implicates the role of ABC transporter proteins in possible chemotherapeutic drug resistance that may vary with the subtype and grade of CMT.
Keywords: ABC Transporter Proteins; BCRP; Canine Mammary Tumour; Drug Resistance proteins; MDR1
Abbreviations: BCRP: Breast Cancer Resistance Protein; CMT: Canine Mammary Tumour; EMT: Epithelial to Mesenchymal Transition; MDR1: Multidrug Resistance Protein 1; ABC: Atp-Binding Cassette.