*Corresponding author:Shreya Shetty, Department of Dentistry program, Ibn Sina National College of Medical Sciences, Jeddah, Saudi Arabia
Received: June 23, 2018; Published: July 13, 2018
To view the Full Article Peer-reviewed Article PDF
Background & Objectives: The purpose of this study was to assess and compare the periodontal treatment outcomes in patients with chronic periodontitis using open flap debridement approach by microsurgical and conventional surgical methods. And to assess and compare the healing outcomes in both the approaches using histopathology&immunohistochemistry also the root surface alterations & presence of residual calculus in the teeth treated by both the methods using scanning electron microscopy (SEM).
Methods: In vitro study design, 5 patients in whom extraction was indicated in teeth in two or more quadrants owing to severe form of periodontitis were selected, one week following surgery, the concerned teeth were extracted with a margin of healing soft granulation tissue around for assessing contents of the healing soft tissue using histopathology & immunohistochemistry and presence of residual calculus and loss of tooth substance using Scanning Electron Microscopy (SEM).
Results: Residual calculus was seen in both the groups. H&E staining showed more hemorrhage in control than test group. Immunohistochemistry revealed staining intensity of smooth muscle action more in test than control group.
Conclusion: The H&E staining examination revealed more areas of hemorrhage in the conventionally treated groups than in the microsurgical groups, but the quality and quantity of fibroblasts could not be assessed in all the sections in both the groups. On the other hand, immunohistochemistry examination revealed that the intensity of smooth muscle actin staining around the endothelium was more prominent in the microsurgery group than in the conventional group which indicates better healing with the use of microsurgery as compared to conventional surgery. The SEM examination revealed residual calculus in both the microsurgically treated and conventionally treated tooth specimens; but loss of tooth substance was more obvious in the conventionally treated specimens in comparison to the microsurgically treated specimens.
Keywords: Microsurgery; Macrosurgery; Open Flap Debridement
Abbreviations: SEM: Scanning Electron Microscopy; EHI: Early wound Healing Index; H&E: Hematoxylin and Eosin