*Corresponding author:Kamal Basri Siregar, Department of Oncology Surgery H Adam Malik Hospital, Medan, Indonesia
Received: June 14, 2018; Published: June 26, 2018
To view the Full Article Peer-reviewed Article PDF
Background: Breast cancer is the one of major cause of death in women especially in developing countries. In 2012 it was recorded nearly 522.000 deaths occurred due to this cancer. Taq 1 polymorphism is a silent SNPs called a genetic risk factor to breast cancer.
The aim of the study: To investigatethe relationship between of vitamin D-Taq1 receptor polymorphism gene (rs 731236) and histopathologic grading in breast cancer patients at Adam Malik Hospital. Medan.
Materials and Methods: Research subjects amounted to 53 breast cancer patients new diagnosed that have not received chemotherapy. DNA isolation from blood cells and gene amplification with PCR followed by RFLP using Taq1 restriction enzyme. Histopathologic grading data was obtained from the patient’s medical records reviewed with anatomical pathologist.
Result: TT genotype was 92.5% genotype TC 7.5%. CC 0% genotype with Hardy-Winberg Equilibrium (HWE) p=0.77.
Histopathologic grading was obtained grade 1 (9.4%), grade 2 (49.1%) and grade 3 (41.5%).
Conclusion: Polymorphism of vitamin D-Taq1 receptor gene was not related to histopathologic grading in breast cancer patients at Adam Malik Hospital (p = 1.000)
Keywords: Breast Cancer TAQ1 Polymorphism, Histopathologic Grading, Vitamin D-Taq1, Histopathologic, Hardy-Winberg, Equilibrium, Exon, Intron, Cardiovascular, Osteoporosis, Cytosine, Nottingham Grading System, Chi-Square, Propensity, Immunohistochemistry, Histopathologic