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ISSN: 2574 -1241
ISSN: 2574 -1241
Received: June 12, 2018; Published: June 19, 2018
DOI: 10.26717/BJSTR.2018.05.001254
The growth of gram-negative anaerobes requires the complete absence of oxygen (≤0.001%), and is usually treated in an anaerobic chamber.However, this traditional method is costly and time consuming.In this study, we describe a new, rapid method for the culture of anaerobic bacteria, such as P. gingivalis, and P. endodontalis, in a rotary shaker under anaerobic conditions, by sealing the bacteria growth media from oxygen exposure using a mineral oil overlay. By using these cells, we found P. gingivalis or P. endodontalis mediated different signaling pathways in RAW264.7 cells.
Keywords: Kinases; Phosphorylation; Menadione; Centrifugation; Proliferation; Adult Periodontitis; Anaerobic Bacteria; Anova; Periodontal Pathogens; Phosphorylation
Abstract| Introduction| Materials and Methods| Results| Discussion| References|
International Association of Landscape Archaeology, Czech Glass Society, Czech Republic
Department of Chemistry, Semenov Institute of Chemical Physics, USSR Academy of Sciences, Moscow, Russia
Pharmaco-kinetics, dynamics and Drug Metabolism, Touro College of Pharmacy, USA
Professor of Nuclear Medicine, Faculty of Medicine and Surgery, University of Milan, Milan, Italy
Clinical Radiologist (MD) - Department of RADIOLOGY, Cosenza Hospital, Cosenza, Italy
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