Research ArticleOpen Access

A New Method for the Cell Culture of Anaerobic Bacteria: P. Gingivalis- or P. Endodontalis-Mediated Pathways in RAW264.7 Cells

Volume 5 - Issue 5

**Xiaoren Tang*, Nujud Zayed H Alamry, Yinian Tang and Serge Dibart**

Author Information Open or Close
- Department of Periodontology, Boston University Goldman School of Dental School, USA
*Corresponding author: Xiaoen Tang, Department of Periodontology, Boston University Goldman School of Dental School, 650 Albany Street, Boston, MA 02118, USA

Received: June 12, 2018; Published: June 19, 2018

DOI: 10.26717/BJSTR.2018.05.001254

Full Text PDF

To view the Full Article Peer-reviewed Article PDF

Abstract

The growth of gram-negative anaerobes requires the complete absence of oxygen (≤0.001%), and is usually treated in an anaerobic chamber.However, this traditional method is costly and time consuming.In this study, we describe a new, rapid method for the culture of anaerobic bacteria, such as P. gingivalis, and P. endodontalis, in a rotary shaker under anaerobic conditions, by sealing the bacteria growth media from oxygen exposure using a mineral oil overlay. By using these cells, we found P. gingivalis or P. endodontalis mediated different signaling pathways in RAW264.7 cells.

Keywords: Kinases; Phosphorylation; Menadione; Centrifugation; Proliferation; Adult Periodontitis; Anaerobic Bacteria; Anova; Periodontal Pathogens; Phosphorylation