info@biomedres.us
+1 (502) 904-2126
One Westbrook Corporate Center, Suite 300, Westchester, IL 60154, USA
Site Map
ISSN: 2574 -1241
ISSN: 2574 -1241
Received: June 12, 2018; Published: June 19, 2018
DOI: 10.26717/BJSTR.2018.05.001254
The growth of gram-negative anaerobes requires the complete absence of oxygen (≤0.001%), and is usually treated in an anaerobic chamber.However, this traditional method is costly and time consuming.In this study, we describe a new, rapid method for the culture of anaerobic bacteria, such as P. gingivalis, and P. endodontalis, in a rotary shaker under anaerobic conditions, by sealing the bacteria growth media from oxygen exposure using a mineral oil overlay. By using these cells, we found P. gingivalis or P. endodontalis mediated different signaling pathways in RAW264.7 cells.
Keywords: Kinases; Phosphorylation; Menadione; Centrifugation; Proliferation; Adult Periodontitis; Anaerobic Bacteria; Anova; Periodontal Pathogens; Phosphorylation
Abstract| Introduction| Materials and Methods| Results| Discussion| References|
International Association of Landscape Archaeology, Czech Glass Society, Czech Republic
Professor of Nuclear Medicine, Faculty of Medicine and Surgery, University of Milan, Milan, Italy
Pharmaco-kinetics, dynamics and Drug Metabolism, Touro College of Pharmacy, USA
Associate Professor at Department of Breast and Thyorid Surgey, Chongqing General Hospital, China
Lecturer at the School of Energy and Chemical Engineering, Xiamen University Malaysia, Malaysia
Full Text
PDF
