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Myocardial Tissue Engineering: A Comparative Study of Different Solutions for Use as a Natural Scaffold Being of Heart a Comparative Study of Different Solutions for Decellularization Heart Scaffold

Volume 5 - Issue 3

Esin Akbay* and Mehmet Ali Onur

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    • Department of Biology, University of Hacettepe, Turkey

    *Corresponding author: Esin Akbay, Department of Biology, Faculty of Science, University of Hacettepe, Ankara, Turkey

Received: May 29, 2018;   Published: June 11, 2018

DOI: 10.26717/BJSTR.2018.05.001191

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Abstract

The purpose of this study is to develop the optimal method for heart decellularization using for tissue engineering. Several different chemical methods have been studied for decellularized tissues including heart. Nevertheless, there are not certain results about which combination of these methods may provide the best transplantable, effective scaffolds. In this study, a new modified decellularization method has been applied to heart including different concentrations of ionic and non-ionic detergents. To observe decellularization results histological analysis were performed. As a result, it was observed that chosen detergent solutions at low percentile concentrations and in short time duration showed efficient performance. Histological analysis indicated that samples were nuclei free in all groups. The results of Acridine orange (AO) and propidium iodide (PI) staining showed that there were a few viable attached cells.Hereby, results demonstrated that obtained tissue scaffolds have a well surface for cell attachment.

Keywords: Decellularization; Heart; Scaffold; Tissue Engineering; Extracellular Matrix

Abbreviation: ECM: Extracellular Matrix; PBS: Phosphate Buffer Saline; AO: Acridine Orange; PI: Propidium Iodide

Abstract| Introduction| Methods| Results| Discussion| Acknowledgment| References|