*Corresponding author:D Mitic Culafic, Faculty of Biology, University of Belgrade, Studentski trg 16, Belgrade, 11000, Serbia
Received: February 02, 2017; Published: April 10, 2018
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The essential fatty acids are one of the vital nutrients to be included in the diet. However, they are susceptible to autoxidation and maintaining their stability during storage is a foremost problem. Synthetic antioxidants used at present are reported to be toxic in nature. Hence, there is a demand from consumers for foods containing natural antioxidants. Various natural products like clove oleoresin, capsicum oleoresin and kalonji seeds are known to show substantial antioxidant activity. Their activity can be improved by studying structural aspects and evaluating their synergism. The current investigation aims to fractionate clove oleoresin by means of column chromatography isolating the eugenol rich fraction. Though clove oleoresin showed pro-oxidant effect, its eugenol rich fraction showed marginal antioxidant activity in sunflower oil. The clove oleoresin and capsicum oleoresin, both showed good antioxidant activity in synergism with kalonji seeds extract. The study indicated the synergistic potential of natural antioxidants to be used as a substitute for synthetic antioxidants.
Keywords: Sunflower Oil; Natural Antioxidants; Clove; Synergistic Activity; Kalonji; Capsicum
Abbreviations:TBHQ: Butylhydroquinone; RSFO: Refined Sunflower Oil; BHT: Butylhydroxyltoluene; BHA: Butylhydroxylanisole; KEE: Kalonji Seeds Ethanol Extract