Short CommunicationOpen Access

Two Alternative Approaches to Generate Live Attenuated Influenza Vaccine Candidates against Potentially Pandemic Avian Influenza H7N9 Virus

Volume 3 - Issue 4

**Ekaterina Bazhenova, Irina Kiseleva*, Isakova-Sivak and Tatiana Kotomina**

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- Federal State Budgetary Scientific Institution Institute of Experimental Medicine (IEM), Saint Petersburg, Russia
*Corresponding author: Irina Kiseleva, Department of Virology, IEM, 12 Acad. Pavlov Street, Saint Petersburg, 197376, Russia

Received: March 26, 2018; Published: April 06, 2018

DOI: 10.26717/BJSTR.2018.03.000925

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Abstract

Avian influenza remains a major pandemic threat. In response to this threat, the objective of our work was the development of live attenuated reassortant influenza vaccine (LAIV) against recent isolates of potentially pandemic H7N9 influenza viruses which may cause serious and fatal disease in humans. Two H7N9 LAIV candidates were generated by using two different approaches: classical reassortment in eggs and reverse genetics. The collected data indicate that regardless of the approach both H7N9 LAIV candidates acquired attenuated properties - temperature sensitivity and cold-adaptation.

Keywords: Pandemic Influenza; Avian Influenza; Live Attenuated Influenza Vaccine; Potentially Pandemic Vaccine

Abbreviations: LAIV: Live Attenuated Reassortant Influenza Vaccine; CA: Cold-Adapted; HPAIV : Highly Pathogenic Avian Influenza Virus; TS: Temperature Sensitive; WHO: World Health Organization; LPAIV: Low Pathogenic Avian Influenza Virus; RG: Reverse Genetics; SPF: Specific Pathogen-Free; WT: Wild Type