Coating of Single Cells towards Biomedical Applications

Sung Ho Yang*

Received: March 19, 2018;   Published: April 05, 2018

DOI: 10.26717/BJSTR.2018.03.000915

Full Text PDF

To view the Full Article   Peer-reviewed Article PDF

Abstract

In 2002, a pioneering work on single cell coating with macromolecules through layer-by-layer (LbL) method was published in Langmuir [1]. Yeast cells were alternately coated with cationic and anionic macromolecules. Even after coating, the cells still survived. Inspired by the work, multilayered macromolecules have been used for coating various types of microbial cells and endospores [2]. Although the survival of microbial cells in the artificial coat was interesting of itself, the impact of works was under evaluated in the biomedical fields because the method was limited to microbial cells and the macromolecular coating had no particular roles. The two limitations have been gradually solved by developing functional coating materials and applying the method to mammalian cells. The function of coating materials was mainly improved by shifting from organic macromolecules to inorganic materials, in other words, from soft to hard materials Tang et al. [3-6]. crystalized calcium phosphate on the multilayer-coated yeast cells, leading to rigid artificial shells [7]. Choi et al. synthesized silica on the multilayer-coated yeast cells in a biomimetic manner, which was the first report on coating living cells with covalent bonded inorganic materials [8-10]. And chrollera cells were coated with Titania using catalytic peptides [11].

Introduction| References|