*Corresponding author:WKSM Abeysekera, Department of Agricultural Technology, University of Colombo, Sri Lanka
Received: March 01, 2018; Published: March 20, 2018
To view the Full Article Peer-reviewed Article PDF
Aim: To evaluate the anti-glycation and glycation reversing potential of fenugreek seed extract.
Methods: Freeze dried 70% ethanolic seed extract of fenugreek was used in this study. Anti-lycation and glycation reversing potential was studied in Bovine serum albumin (BSA)-glucose model. Antioxidant properties were studied using total polyphenolic content, total flavonoid content, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS),1,1-diphenyl-2-picrylhydrazine (DPPH) and ferric reducing antioxidant power (FRAP) in vitro antioxidant assays.
Results: The results demonstrated significant (P < 0.05) and dose dependant anti-glycation [IC50: (147.99±3.94) μg/mL] and glycation reversing activities [EC50:(553.37±8.02) μg/mL] and marked antioxidant properties [total polyphenolic content: (43.67±2.85) mg gallic acid equivalents/g of extract; total flavonoid content: (25.25±0.91) mg quercetin equivalents/g of extract; 2,2’-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazine (DPPH) and ferric reducing antioxidant power (FRAP): (35.45±0.32), (28.13±0.76) and (21.06±0.42) mg Trolox equivalents/g of extract, respectively].
Conclusion: Seed extract of fenugreek had both anti-glycation and glycation reversing activities in BSA-glucose glycation model. Glycation reversing activity of fenugreek seed extract is a novel finding having therapeutic potentials. Thus, findings of this study indicate usefulness of fenugreek seed in managing advanced glycation end products associated pathologies in diabetes.
Keywords: Anti-Glycation; Glycation Reversing; Antioxidant Activity; Advanced Glycation End Products; Diabetes Complications; Fenugreek; Seed Extract
Abbreviations: BSA: Bovine Serum Albumin; FRAP: Ferric Reducing Antioxidant Power; DPPH: Diphenyl Picrylhydrazine; ABTS: Azino-Bis Ethylbenzothiazoline Sulfonic Acid; AGEs: Advanced Glycation End Products; TPC: Total Polyphenolic Content; GAC: Gallic Acid Equivalents; TE: Trolox Equivalents; DMRT: Duncan’s Multiple Range Test