Pharmacological Analysis of the Potentiation of Carrageenin-Induced Paw Oedema by Reactive Blue 2

Stefano Evangelista*

Received: February 13, 2018;   Published: February 20, 2018

DOI: 10.26717/BJSTR.2018.02.000780

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Abstract

Reactive blue 2 is an a specific antagonist of the ATP P2X and P2Y receptors that are ligand-gated ion channels widely distributed in the organism and that serve as control of excitability of their host cells. Due to the known involvement of ATP in inflammatory and pain processes, we have evaluated the pro-inflammatory effect of reactive blue 2 in the paw oedema induced by carrageenin in rats. Reactive blue 2 did not by itself induce paw oedema but enhanced all phases of the inflammatory response induced by carrageenin. In the first phase of the oedema (0-1 h), compound 48/80, but not the serotonin and histamine receptor antagonists cimetidine, cyproeptadine and diphenydramine, counteracted the pro-oedema effect of reactive blue 2. The second phase (0-2 h) of carrageenin+reactive blue 2 was markedly antagonized by hexadimethrine, a heparin antagonist. The late phase, characterized by prostaglandins involvement was partly antagonized by the dual antagonist of cycloxigenase and lipoxygenase BW 755 and by dexamethasone, but not by indomethacin. The modulating effect of reactive blue 2 on ATP via other autacoids such as heparin is probably responsible on its pro-inflammatory effects.

Keywords: Adenosine-5’-Triphosphate (ATP); Carrageenin; Inflammation; Reactive Blue 2

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