*Corresponding author:Young Wook Jo, Department of Biotechnology, College of Engineering, Yonsei University, Seoul 03722, Republic of Korea, Email: email@example.com
Received: December 14, 2017; Published: February 13, 2018
To view the Full Article Peer-reviewed Article PDF
We prepared a bone plate enabled with the local, sustained release of BMP2, which is a drug known to inhibit Osteoclastic-mediated bone resorption and also expedite the bone-remodeling activity of osteoblasts. For this, we coated a bone plate already in clinical use (PLT-1031, Inion, Finland) with a blend of BMP2 and a biocompatible polymer, 4-azidobenzoic acid-modified chitosan (i.e., Az-CH) photo-cross linked by UV irradiation. As we performed the in vitro drug release study, the drug was released from the coating at an average rate of 4.03μg/day for 63 days in a sustained manner. To examine the effect on bone regeneration, the plate was fixed on an 8mm cranial critical size defect in living rats and the newly formed bone volume was quantitatively evaluated by micro-computed tomography (micro-CT) at scheduled times over 8 weeks. At week 8, the group implanted with the plate enabled with sustained delivery of BMP2 showed a significantly higher volume of newly formed bone (52.78 ± 6.84%) than the groups implanted with the plates without drug (23.6 ± 3.81%) (p b 0.05). The plate enabled with BMP2 delivery also exhibited good biocompatibility on H&E staining, which was comparable to the Inion plate already in clinical use. Therefore, we suggest that a bone plate enabled with local, sustained delivery of BMP2 can be a promising system with the combined functionality of bone fixation and its expedited repair.
Abbreviations: PLGA: Poly Lactic-Co-Glycolic Acid; PLA: Poly Lactic Acid; PGA: Poly Glycolic Acid; FDA: Food and Drug Administration; ACDF: Anterior Cervical Discectomy and Fusion; PEI: Poly Ethylenimine; PEG: Poly Ethylene Glycol; SEM: Scanning Electron Microscopy; UTS: Ultimate Tensile Strength; UTM: Universal Testing Machine; ELISA: Enzyme-Linked Immunosorbent Assay; DMEM: Dulbecco’s Modified Eagle Medium