Effect of Local Administration of Voltage-Gated Calcium Channels Blocker (Gallopamil) Combined with Silicon Conduit on Transected Sciatic Nerve

Parvin Zareian¹, Minoo Shaddel², Mohammad Velayati¹*, Hamid Sahamsi¹ and Rahim Mohammadi³

Received: August 31, 2017;   Published: September 13, 2017

DOI: 10.26717/BJSTR.2017.01.000355

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Abstract

Background: The outgrowth of regenerating axons of a transected peripheral motor nerve is a slow process. The objective was to assess the effect of locally administered gallopamil on transected peripheral nerve regeneration and functional recovery.

Methods: Sixty male healthy white Wistar rats were divided into four experimental groups (n = 15), randomly: In transected group (TC), left sciatic nerve was transected and stumps were fixed in the adjacent muscle. In treatment group defect was bridged using silicone tube (SIL/ Gallopamil) filled with 10 μL gallopamil (100 ng/mL). In silicone conduit group (SIL), the tube was filled with phosphate-buffered saline alone. In sham-operated group (SHAM), sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups of five animals each and regenerated nerve fibers were studied 4, 8 and 12 weeks after surgery.

Results: Behavioral testing, biomechanical studies, sciatic nerve functional study, gastrocnemius muscle mass and morphometric indices confirmed faster recovery of regenerated axons in SIL/Gallopamil than SIL group (p < 0.05). In immunohistochemistry, location of reactions to S-100 in SIL/Gallopamil was clearly more positive than that in SIL group.

Conclusion: When loaded in a silicone tube gallopamil accelerated and improved functional recovery and morphometric indices of sciatic nerve. This may have clinical implications for the surgical management of patients after nerve transaction.

Keywords: Peripheral Nerve Repair, Sciatic, Gallopamil, Local

Abbreviations: TC: Transected Group; SIL: Silicone Conduit Group; SHAM: Sham Operation Group; BBB: Basso, Beattie and Bresnahan; SFI: Sciatic Functional Index; N-CAM: Nerve Cell Adhesion Molecule

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