Applications of Mass-Spectrometry Based Quantitative Proteomics to Understand Complex Cellular Functions and Cell Fate Decisions

Sajib Chakraborty and Hossain Uddin Shekhar*

DOI: 10.26717/BJSTR.2017.01.000144

Received: June 08, 2017   Published: June 19, 2017

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Abstract

Unlike the transcriptome, quantification of the proteome still requires the development of special strategies. Transcriptomics platforms such as microarray- and RNAseq-based approaches are designed to uncover the information at the transcriptome level that in turn shapes the proteome to carry out the functional cellular processes. Since most of the biological processes are controlled by proteins, it is important to quantitatively measure proteome alterations in varying conditions to mechanistically understand how cellular processes are carried out. Within the last decade mass spectrometry increasingly became the method of choice for proteome analyses and now also provides a solid platform for its accurate quantification. The aim of quantitative proteomics is to obtain reliable quantitative information about all the proteins that fall within the mass spectrometric dynamic range.

Mass Spectrometry-Based Proteomics | Mass Spectrometry-Based Proteome Quantification Methods - Overview | Conclusion | References | Figures | Tables |