Yellow Wasp Polistes Flavus Venom Protein, Its Purification, Solubilization and Antimicrobial Activity

Krishna Kumar Prajapati and Ravi Kant Upadhayay*

DOI: 10.26717/BJSTR.2017.01.000141

Received: June 06, 2017   Published: June 19, 2017

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Abstract

Yellow wasp Polistes flavus venom toxins were isolated by cutting the last two segments of abdominal region and purified on Sepharose CL- 6B 200 column, the molecular weight of venom protein from yellow wasp ranging from 6-70kD. The purified venom toxins were investigated for its antimicrobial activities against three infectious bacterial pathogens. Paper disc diffusion and serial dilution assays were performed for determination of Inhibition Zone Diameter (IZD) and Minimal Inhibitory Concentration (MIC) respectively. MIC values obtained were 12.3 μg/ ml against E. coli, and 24.69 μg/ml for Salmonella typhi and Vibrio cholera. By agar disc diffusion method, the Inhibition Zone Diameter (IZD) for E. coli, Salmonella typhi and Vibrio cholera were 36±0.14, 19.96±0.36 and 21.69±0.22 respectively. In present investigation, Triton X-100 was found good solubilizing agent for venom protein. The antibacterial effects of wasp venom may be due to the action on cell membrane destruction and cell-lyses.

Keywords: Polistes flavus; Venom protein; Inhibition Zone Diameter; Antibacterial activities and peptides

Abbreviations: AMP: AntiMicrobial peptides; PCD: Programmed Cell Death; PDVs: PolyDnaViruses

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