Abstract

124-Iodine (¹²⁴I) is an attractive radionuclide in clinical and preclinical PET imaging studies with a long half-life of 4.2 days. ¹²⁴I has specific physical characteristics that make it more suitable than the more conventional PET radionuclides. The aim of this study is to evaluate some quantitative imaging characteristics of ¹²⁴I-PET using a small animal Nano Scan PET/CT system and comparing these results with those obtained for ¹⁸F and ⁸⁹Zr.

Methods: The system's spatial resolution, uniformity, and image quality were measured on a Nano Scan small-animal PET/CT scanner according to the NEMA NU4-2008 protocols. For reconstruction images, we used 2D and 3D reconstruction algorithms. The reconstruction methods included filter back projection (FBP)and the 3D Tera-Tomo algorithm, which are developed for the NanoScan small-animal PET/CT scan.

Result: The long mean positron ranges of ¹²⁴I (3.48 mm) caused a significantly low resolution (high value of FWHM) as compared with ⁸⁹Zr and ¹⁸F, it was 1.7mm for¹²⁴I where this value was 0.9 and 1 mm for ¹⁸F and ⁸⁹Zr, respectively. The spillover ratio (SORs) values were the highest value in water filled compartment for ¹⁸F, while they were close to each other for ¹²⁴I and ⁸⁹Zr. On the other hand, in the case of air filled compartment the results have shown no significant difference. The results of recovery coefficient (RC) values have shown a significant reduction was observed for ¹²⁴I. The high differences in the SOR and significant reduction in RCs were observed for ¹²⁴I as compared to ⁸⁹Zr and ¹⁸F. Best results were found for ¹⁸F.

Conclusion: In this study the image quality parameters and spatial resolution were evaluated for ¹²⁴I using small animal NanoScan® PET/CT scanner. We have considered the main problems that can limit the image quality performance is the higher positron range and the presence of prompt ɣ-photons. The presence of ɣ-photons effects on the image quality parameters for high positron range, particularly SD%.

Introduction

In recent years, PET/CT is considered as an essential multimodality imaging that commonly used in oncology due to its ability to providing both anatomical and functional information. PET/CT has an important value in the detection and staging of several types of cancer. It can improve cancer diagnosis and follow-up the assessment of tissues response to treatment. The PET/CT imaging can help in detection of small lesions. These developments are showing dramatically in the advance generations of PET/CT which include new software, hardware, and acquisition methods [1], for example it introduces some new image reconstruction methods such as time –of- flight (TOF), a continuous bed motion and combined PET with other modalities. These growths in PET/CT can influence on cancer imaging as the can produce better image quality and more accurate details of therapy monitoring and uptake [1]. Fluorine -18 (¹⁸F) is considered as a gold standard positron emitter in PET imaging, it has a widely uses in imaging applications when it is labelled with some pharmaceutical compounds such as ¹⁸F-fluorodeoxyglucose (FDG),¹⁸F-fluoromisonidazole,¹⁸F-fluoroazomycin-arabinoside, and ¹⁸F-fluoro-3'-deoxy-3'-L-fluorothymidine. Nowadays, PET imaging plays an important role in radioimmunotherapy (RIT) which known as Immuno-PET and it is used to estimate cancer treatment with monoclonal antibodies (mAbs) [2].

The best radionuclides for these applications should has a physical half-life matches the biological half-life of antibodies (mAbs) or pharmaceutical molecules in circulation, so that other positron emitters with half-life greater than 48h are being used such as ¹²⁴I ,⁸⁹Zr and Y90 [3]. Each of these isotopes has different physical characteristics for instance positron energies, positron ranges and fraction of single photons emitted [3]. The long physical half-life of I-124 which is about 4.2 days make it suited in vivo studies. Despite this optimal characteristic for matching with mAbs, two main features can be expected to limit the image resolution in in preclinical PET imaging, one is the high energy and therefore long range of the emitted positrons. Pre-clinical imaging on animals are critical tools to explore multi-scale variations i.e. from organ, tissue, cell, down to molecular levels, resulted due to physiological, pathological or pharmacological changes, Precedes to human study human clinical trials. Rapid development in PET systems can permit the translation of large scale PET imaging (which used in human and large animals) abilities to the scale of small animals by using small-animal PET [4]. The use of small animal imaging has become an effective key in translational research, it is considered as a bridge between developments in vitro studies and clinical applications in diagnosis and/or therapeutics [5].

Some of disadvantages include the high positron energy and large positron range in tissue (e.g., ⁶⁸Ga and ¹²⁴I) may effect on the spatial resolution of the image and produce low spatial resolution and recovery coefficient (RC). Similarly, the presence of single ɣ photons (e.g., ¹²⁴I and ⁸⁹Zr) can have undesirable effects on the image quality, it might increase image noise and spillover ratios (SORs). The purpose of this study was to compare some of performance parameters such as image quality and spatial resolution of ¹⁸F, ¹²⁴I and ⁸⁹Zr using small animal Nano-Scan PET/CT.

Materials and Methods

PET/CT Scanner

The NanoScan PET/CT system is used (Mediso Ltd) (Figure 1), which has a high sensitivity, high resolution and fully integrated small-animal PET/CT system. The axial field-of-view (FOV) of the PET ring is 10 cm. The PET system has 12 detector blocks of 81×39 Lutetium Yttrium Orthosilicate (LYSO) crystals (1.12 mm×1.12 mm×13.0 mm), tightly packed (pitch 1.17 mm, packing fraction 92%) coupled to two 256-channel position sensitive photomultiplier tubes. The detector blocks are mounted in rings of diameter ¹⁸4 mm, and there are 2 rings in total. Each detector block is connected with 1, 3, or 5 blocks on the opposite direction of the ring for coincidence detection (coincidence mode 1:1, 1:3 and 1:5). The CT system is a cone-beam CT scanner. It has a flat panel CMOS detector of 15×12 cm² Gd₂O₂S. The maximum current of X-ray tube is 0.18 mA, the tube voltage ranges from 30 to 80 kVp and the tube power is 50/80 W. PET data are sorted in list mode format and for image reconstruction several options can be used; filtered Back-projection (FBP), 3D projection, 2D and 3D ordered-subset expectation maximization (OSEM2D and OSEM3D, respectively). A fully 3-dimensional (3D) reconstruction using a Tera-Tomo algorithm (Tera-Tomo; Mediso Ltd.), all image quality factors are achieved for at least 30 min acquisition and image reconstruction with 52 iterations which is the default image reconstruction method of NanoScan PET/CT, also used. A multiple Graphical Processing Unit (GPUs) are used for CT reconstruction. Reconstruction is done using filtered back-projection method, with following available filters; RamLack, Hamming, Hann, Shepp-Logan, Butterworth, Cosine and BlackMan.

Radionuclides

The radionuclides used in this study are ¹⁸F ¹²⁴I and ⁸⁹Zr. The main physical properties of these radionuclides are present in (Table 1). ¹⁸F is most commonly used in PET scan which is used for early detection of tumors and evaluation of response to cancer therapy. Moreover,¹⁸F is an essential isotope for standard measurements of PET acceptance tests suggested by NEMA NU. Today, the two others radionuclides, ¹²⁴I and ⁸⁹Zr, are increasingly used in PET. ¹²⁴I is a radionuclide with positron abundance of approximately 23%, about 50% of all positrons are emitted simultaneously with a 603-keV gamma photon a high positron energy and a high abundance of singles. ¹²⁴I has dual energy emission: beta radiation emissions of 1532 keV (11%) and 2135 keV (11%) and gamma emissions of 511 keV (46%), 603 keV (61%), and 1691 keV (11%). ⁸⁹Zr is a neutron deficient isotope of Zirconium with 49 neutrons and 40 protons, and it decays with a half-life of 3.27 days to ⁸⁹Y. The decay proceeds via electron capture (77%), and positron emission (23%). Both modes of decay lead primarily (99%) to a 9/2+ state in ⁸⁹Y, which de-excites through M4 emission of a 909 keV gamma ray to the 1/2- ground state. Therefore, the important decay radiations are the 511 keV γ’s from positron annihilation, the 909 keV γ from the ^89mY de-excitation, continuum positrons (23% endpoint = 902 keV), internal conversion electrons from the M4 transition (0.8%), and Auger electrons. The prompt photons fall outside the default energy window, apart from a portion of the down-scattered photons with lower energy.

Their high yield (0.99 ɣ-photons vs. 0.23 positrons) could contribute to the detector dead time [6]. Radionuclide production was carried out at King Faisal Specialist Hospital and Research Centre. ⁸⁹Zr was produced through the ⁸⁹Y (p, n) ⁸⁹Zr reaction by proton bombardment of ⁸⁹Y solid targets. Enriched Y2O3 targets in oxide form were prepared by hydraulically pressing (130–200 mg) ⁸⁹Y oxide powder (130–200 mg) into circular cavity aluminum target plates at 5000 psi. The Y₂O₃ targets were irradiated with accelerated protons at 90° to the cyclotron beam using a CS30 cyclotron (CTI Cyclotron Systems, Berkeley, California, USA) [4]. Irradiation times were between 2 and 3 h, energy was 15 MeV, beam current was 10 μA, and the ⁸⁹Zr yield obtained was around 8mCi. ⁸⁹Zr was isolated with high radionuclide and radiochemical purity (>99%) as ZrCl₄ using a solid-phase hydroxamate resin with more than 95% recovery of the radioactivity [4]. 24I was produced by the bombardment of enrich ¹²⁵Te targets (150 mg ±10%) with 18 MeV protons from the IBA Cyclon 30 using ¹²⁵Te(p,n)¹²⁴I nuclear reaction. The targets were prepared and processed by a standard operating procedure developed in-house. In brief, irradiated targets were dissolved in a mixture of sodium hydroxide (NaOH, 1.0 mL, 5.0 N), hydrogen peroxide (H₂O₂, 3.0 mL, 30 %) and de-ionized water (6.0 mL).

The dissolved ¹²⁵Te was transferred in 250 mL quartz reaction flask containing aluminum powder (160 mg) which was boiled until reaction is completed. Air was then bubbled through the solution for five minutes followed by bubbling of CO₂ for additional five minutes. The bubbled solution was then carefully filtered through a fine fritted glass and on-line AG50W-X8 column into pre-weighed serum vial. If necessary, the pH was adjusted in the range of 5-7 with NaOH (0.01 N). Finally, 0.6 mL of the Na¹²³I bulk solution was subjected to quality control measures for radionuclidic purity, specific concentration, pH, tellurium and aluminum spot tests. Extracted ¹²⁴I was used immediately for radiopharmaceutical production [7].

Spatial Resolution

The NEMA NU-2004 is specified to evaluate the spatial resolution for ²²Na only. For this reason, the transaxial spatial resolutions of the three radionuclides were measured using a new suggested method by us. For measuring the spatial resolution of ¹⁸F and ⁸⁹Zr a point source was used. It was obtained by drilling a plastic plate to make a sphere with a radius less than 1 mm, and then it was filled with different isotopes. The activity was 3.37 MBq, 8.3 MBq and 0.32 MBq for ¹⁸F, ¹²⁴I and ⁸⁹Zr, respectively, this difference in doses of isotopes is due to an availability issue. The activity was positioned in the center of the FOV, aligned with the axis of the scanner, and then put at 10 mm and 20 mm and scanned until at least 3.5 million counts were acquired using the default energy window of 250–750 keV and timing window of 3.432 ns. Images were obtained using FBP and the 3D Tera-Tomo algorithm, which are developed for the NanoScan small-animal PET/CT scanner with the default settings (ramp filter with a cutoff at Nyquist frequency) and 512 ×512 ×159 matrices with voxel sizes of 0.1 × 0.1×0.58 mm. This matrix size was selected to obtain a profile with a sufficient number of data points to allow for the accurate determination of spatial resolution all image quality factors is achieved for at least 30 min acquisition and image reconstruction with 52 iteration . No correction was applied, whether attenuation or scatter corrections. The reconstructed images were processed to measure full width at half and at tenth maximum (FWHM and FWTM, respectively) for the point source. According to the NEMA NU-4 standards, these widths were measured in parabolic fit through the peak pixel and its two nearest neighboring pixels. FWHM and FWTM were not corrected for source geometry [2].

Image Quality

The image quality tests were done using NEMA image quality phantom (Figures 2 & 3), it is divided into three parts: the first region is a homogeneous block filled with radioactivity (30-mm diameter and 30-mm length) to determine the signal-to-noise ratio of the system. The second one consists of two chambers filled with cold water and air (length, 15 mm; outer diameter, 10 mm; wall thickness, 1 mm) to evaluate the scatter fraction in the image. The third part is a 5-rod region, these rods are used to calculate the recovery coefficient (RC) which is defined as the ratio between the measured activity concentration in the rods and the activity concentration measured in the uniform area [8]. The RC is theoretically limited between 1 and 0 (0 < RC < 1). A 2-cylinder-chamber region, one is filled with air, the other was filled with nonradioactive water, are used to measure the spillover ratio (SOR) in water and air [2], which defined as the mean value in each cold cylinder divided by the mean in the uniform area and its theoretically value is range between 0 and 1 [2]. The percentage SD% which is considered as a measure of noise was determined by using the uniform region at the central of phantom. The phantom was filled with a specific isotope and the injected activity was 3.7 MBq. The scanning time was 20 minutes, a 5-ns time window and a 400-to-600 keV energy window. The measurement was performed in the normal-count mode of the scanner in 1:3 and 1:5 coincidence modes. In addition to the 2D-OSEM reconstruction image, the acquired data were reconstructed with default reconstruction algorithm of the system, Tera-Tomo reconstruction method. The image quality tests include; uniformity, recover coefficients and accuracy of correction.

Results

In the following sections, the results for spatial resolution and image quality parameters are presented. The obtained values of three radionuclides are summarized in (Table 2).

Spatial Resolution

The spatial resolution measurements using a point source of each radionuclide reconstructed with a Tera-Tomo 3D PET algorithm and with FBP-reconstruction image are presented in (Table 2). The spatial resolutions of tangential and radial direction at the center were averaged to calculate FWHM and FWTM. The FWHM were 0.9 mm, 1.0 mm and 1.7 mm for ¹⁸F, ⁸⁹Zr and ¹²⁴I, respectively, when using 3D-reconstruction method. While the spatial resolution values were lower when used FBP-reconstruction method, the FWHM values were 2.0 mm, 2.0 mm and 2.6 mm for ¹⁸F, ⁸⁹Zr and ¹²⁴I, respectively. According with Gaussian profile, the FWHM to FWTM ratio equal to = 0.55, nongaussian profiles with extended tails are characterized by smaller FWHM-to-FWTM ratios [2]. Palmer, et al [9] and Disselhorst, et al [2] have measured spatial resolution and determined the corresponding FWHM to FWTM ratios, and they have showed that ¹⁸F have produced the best value of spatial resolutions and theoretic ratios (0.54 and 0.51 respectively). The data in Table 2 show that the

values of spatial resolution improve when using Tera-Tomo algorithm more than those in FBP algorithm for all isotopes, these values were 0.9, 1.0,and 1.7 mm for ¹⁸F, ⁸⁹Zr and ¹²⁴I, respectively. In addition, these data indicate that ¹⁸F provided the best values as compared to other isotopes (0.45) where the measured ratio of ⁸⁹Zr (0.43, 0.5 and 0.5%, respectively) and for ¹²⁴I (0.4, 0.39 and 0.41% respectively) are lower and we observed the same effect when we use 2D reconstruction (see Tables 2 & 3).

Image Quality Results

Uniformity

The %SD of the NEMA Nu-4 image-quality phantom is shown in (Figure 3). The largest differences are not obtained between ¹⁸F and ⁸⁹Zr but it seems clear with¹²⁴I. Comparable values were obtained with F-18 and Zr-89, however I-124 showed a 47% higher value. For ¹²⁴I, the % SD is greater than the values that were found for ¹⁸F and ⁸⁹Zr. Moreover, among the different reconstruction algorithms for ¹²⁴I there was a significant increase, where Tera-Tom yielded %SD value smaller than those obtained by FBP, it has shown 47% increase than those obtained by FBP.

Accuracy of Corrections

The spill-over ratio (SOR) in cold regions is calculated to find the accuracy of activity correction. The spill-over ratio and STD % in the water- and air-filled cylinders are given in (Table 3). These results are obtained for 2D OSEM and 3D Tera-Tom. The difference in SOR in water was large between¹⁸F and¹²⁴I, it was about 0.88. ¹⁸F has higher SOR value when used Tera-Tomo algorithm or FPB algorithm, it was 1.0 and 0.95, respectively, while ⁸⁹Zr and ¹²⁴I have lower SORs values than ¹⁸F. For ⁸⁹Zr, we found a reduction in SOR value when use Tera-Tom algorithm, it was 0.16 where it was about 0.2 when using FPB algorithm. These values were 0.12 and 0.16 respectively, for ¹²⁴I. The difference in SORs in air field chamber are shown in (Figure 4a). (Figure 4b) illustrated the effect of the image reconstruction algorithms on the SORs values for each radionuclide. There were no significant variations in SORs values between ¹⁸F ,⁸⁹Zr and ¹²⁴I when used Tera-Tomo reconstruction method, the SORs values were 0.15,0.17 and 0.16 for ¹⁸F ,⁸⁹Zr and ¹²⁴I, respectively. In the case of using FBP reconstruction algorithm, the SORs values were increased for ¹⁸F and⁸⁹Zr, they were 0.23 for both ¹⁸F and⁸⁹Zr where the SOR value of ¹²⁴I decreased by 52% as compared with ¹⁸F and ⁸⁹Zr, it was 0.11 for ¹²⁴I (Table 4). The attenuation and scatter correction were not used because it did not install on the system.

RCs

The RC values and (STD%) using 3D and 2D reconstruction are summarized , (Figure 5) represents the data. A significant difference was introduced for different radionuclides. The smallest values of RC were found for ¹²⁴I as compared to ¹⁸F and ⁸⁹Zr, but this variation not obtained for different reconstruction algorithms. A high reduction in RC value was observed for ¹⁸F when used FBP reconstruction. ⁸⁹Zr has the highest value of RC in both cases of different reconstruction algorithms (Figure 6). The maximum difference was shown for 3 mm rod. In all results that discussed above, no attenuation or scatter corrections were used.

Discussion

Several studies have studied the effective parameters on the image quality of some long-half-life positrons, such as ⁸⁹Zr, ¹²⁴I, and ⁶⁸Ga. This study looks at the imaging properties of ¹²⁴I and compares them to those of ¹⁸F when it is used with a small animal NanoScan® PET/CT scanner. As mentioned in the Lubberink study [10], positron energy is one of the most important factors affecting image resolution. He showed that ¹²⁴I emits positrons with energies ranging from 686 to 973 Kev, while ¹⁸F emits positrons with a mean energy of 250 Kev and 89Zr with 389 Kev. This increase in positron energy of ¹²⁴I results in a reduction in spatial resolution. On the other hand, The greater positron ranges of ¹²⁴I, on the other hand, have an effect on spatial resolution, which is more severe in small animal PET than in human whole-body PET. The theoretical spatial resolution for small animal PET is 1.3 mm and 2.9 mm for ¹⁸F and I-124, respectively [10]. As expected, the results of the point source are consistent with previous studies. The positron range limits the spatial resolution in pre-clinical systems [3]. ¹²⁴I, with a mean positron range of about 3.48 mm, has significantly low resolution (high value of FWHM) as compared with ⁸⁹Zr and ¹⁸F. There was a 47.6% reduction. This is consistent with other studies, such as Liu and Laforest's [11] study, which evaluated the resolution of 76Br using designed phantoms in a high-resolution small animal PET scanner and found that 76Br can achieve 2 mm of spatial resolution while ¹⁸F can only achieve 1.7 mm.

De Jong, et al. [11] have analyzed the imaging characteristics of ⁶⁸Ga (Emax = 1.9 MeV, + range = 3.1 mm), ¹²⁴I (Emax = 2.1 MeV, + range = 3.0 mm), and ⁸⁹Zr (Emax = 0.9 MeV, + range = 1.23 mm), and they found that ¹⁸F has the best resolution of all isotopes investigated, followed by ⁸⁹Zr, because of their relatively shorter positron range as compared to ⁶⁸Ga and ¹²⁴I [12]. Additionally, a study by Lee, et al. [13] showed that the spatial resolution of ¹²⁴I was degraded by 19.9% compared to that of ¹⁸F on the ECAT HR+ scanner (Siemens Healthcare GmbH). Alanazi, et al. [4] have shown that there was a 2.2% decrease in spatial resolution measured at 1 cm off-axis for ⁸⁹Zr in comparison with the obtained value of ¹⁸F Radial and tangential spatial resolution values measured at 10 cm off-axis have 2 and 4.1% reductions for ⁸⁹Zr in comparison with ¹⁸F, respectively. This shows that ¹⁸F has a significantly higher resolution than ⁸⁹Zr. The SOR values were the highest values in the water-filled compartment for ¹⁸F, while they were close to each other for ¹²⁴I and ⁸⁹Zr. On the other hand, in the case of an air-filled compartment, the results have shown no significant difference when Tera-Tomo 3D reconstruction was used. This may be because we did not use any correction, scatter, or attenuation. This can have caused the positrons emitted in the body part of the phantom to annihilate in the water-filled scatter compartment. The scatter and single photon have the main contribution to the SORs [4].

In a perfect system, the optimal limit of the recovery coefficients (RC) is supposed to equal 1. For both isotopes, the RCs discovered in this test had lower RC values when reconstructed using the 2D reconstruction algorithm than when reconstructed with the 3D reconstruction algorithm. For F-18 utilizing 2D, the RC values were approaching 1 for rods with a long diameter of 3 mm, 4 mm, and 5 mm, and about 1 for Zr-89. While for rods with diameters of 3 mm, 4 mm, and 5 mm, the RCs values determined using the 3D reconstruction algorithm exceeded 1 for both F-18 and Zr-89. When employing the 2D and 3D reconstruction algorithms, there was no significant difference in the RC values of I-124. This effect is known as the Gibbs effect [14]. These RC values match those published by Nagy [15] for NanoScan PET/MRI system rods with diameters of 1 mm and 2 mm. According to Goertzen [16], the RC values are highly dependent on the reconstruction algorithm. In addition, the prompt ɣ-photons for ¹²⁴I and ⁸⁹Zr have contributed to image noise and caused an increase in STD%. This appeared clearly in ¹²⁴I. The results of RC values have shown the effect of positron range. A significant reduction was observed for ¹²⁴I. These results are compatible with those obtained by Liu, et al. [17] where they found the RCs values of radionuclides which have a longer positron range, such as 61Cu and ⁶⁸Ga, than ¹⁸F, were lower than the RCs values of ¹⁸F. The corrected RCs in medium and smallest spheres ranged from 75% to 90% for 61Cu and ⁶⁸Ga, where s for ¹⁸F are close to 100%. Lubberink, et al. [10] investigated the effect of image resolution degradation for ¹²⁴I on recovery, and they showed that recovery for ¹²⁴I was considerably worse than for ¹²⁴I, even for spheres as large as 37 mm in diameter.

Conclusion

In this study, the image quality parameters and spatial resolution were evaluated for nonconventional radionuclides such as ¹²⁴I and ⁸⁹Zr using the small animal NanoScan® PET/CT scanner. The presence of prompt ɣ -photons and the higher positron range are two major issues that can limit image quality performance. The high differences in the SOR and significant reduction in RCs were observed for ¹²⁴I as compared to ⁸⁹Zr and ¹⁸F. The best results were found for ¹⁸F. The presence of ɣ -photons effects on the image quality parameters for the high positron range, particularly SD%. Due to the no-scatter correction being used, the single photon can contribute and cause some artifacts in the image. These artifacts can cause distortions in SOR and RC values.

Disclosure

No potential conflict of interest relevant to this article was reported.

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ABSTRACT

124-Iodine (¹²⁴I) is an attractive radionuclide in clinical and preclinical PET imaging studies with a long half-life of 4.2 days. ¹²⁴I has specific physical characteristics that make it more suitable than the more conventional PET radionuclides. The aim of this study is to evaluate some quantitative imaging characteristics of ¹²⁴I-PET using a small animal Nano Scan PET/CT system and comparing these results with those obtained for ¹⁸F and ⁸⁹Zr.

Methods: The system's spatial resolution, uniformity, and image quality were measured on a Nano Scan small-animal PET/CT scanner according to the NEMA NU4-2008 protocols. For reconstruction images, we used 2D and 3D reconstruction algorithms. The reconstruction methods included filter back projection (FBP)and the 3D Tera-Tomo algorithm, which are developed for the NanoScan small-animal PET/CT scan.

Result: The long mean positron ranges of ¹²⁴I (3.48 mm) caused a significantly low resolution (high value of FWHM) as compared with ⁸⁹Zr and ¹⁸F, it was 1.7mm for¹²⁴I where this value was 0.9 and 1 mm for ¹⁸F and ⁸⁹Zr, respectively. The spillover ratio (SORs) values were the highest value in water filled compartment for ¹⁸F, while they were close to each other for ¹²⁴I and ⁸⁹Zr. On the other hand, in the case of air filled compartment the results have shown no significant difference. The results of recovery coefficient (RC) values have shown a significant reduction was observed for ¹²⁴I. The high differences in the SOR and significant reduction in RCs were observed for ¹²⁴I as compared to ⁸⁹Zr and ¹⁸F. Best results were found for ¹⁸F.

Conclusion: In this study the image quality parameters and spatial resolution were evaluated for ¹²⁴I using small animal NanoScan® PET/CT scanner. We have considered the main problems that can limit the image quality performance is the higher positron range and the presence of prompt ɣ-photons. The presence of ɣ-photons effects on the image quality parameters for high positron range, particularly SD%.