"Quantification of Flavonoides and Phenols of Bravo Bean Extract (Capparis flexuosa L. Caparaceae)"

The use of plants for medicinal purposes is not present only in contemporaneity, since the course of human history there was already the search for plants that possessed the curative capacity, even with little knowledge about them, the men of antiquity already used them and distinguished them for the various purposes. Medicinal herbs are fundamental in the treatment of various pathologies, especially in communities isolated from large urban centers [1]. With the advent of navigations it was possible to discover new continents, presenting to the world the great therapeutic arsenal derived from plants, indispensable to modern medicine [2]. Nature has always been a source of charm for man, not only for the useful resources of its food and maintenance, but for being its greatest source of learning and inspiration [3]. Brazil is a privileged country in biodiversity since, of all the plant species in the world, 20% is in The Brazilian territory. Several native or nonnative species face risk of extinction due to the degradation of their habitat and exploratory [4].


Introduction
The use of plants for medicinal purposes is not present only in contemporaneity, since the course of human history there was already the search for plants that possessed the curative capacity, even with little knowledge about them, the men of antiquity already used them and distinguished them for the various purposes. Medicinal herbs are fundamental in the treatment of various pathologies, especially in communities isolated from large urban centers [1]. With the advent of navigations it was possible to discover new continents, presenting to the world the great therapeutic arsenal derived from plants, indispensable to modern medicine [2]. Nature has always been a source of charm for man, not only for the useful resources of its food and maintenance, but for being its greatest source of learning and inspiration [3]. Brazil is a privileged country in biodiversity since, of all the plant species in the world, 20% is in The Brazilian territory. Several native or nonnative species face risk of extinction due to the degradation of their habitat and exploratory [4].
Although Brazil has a great biodiversity, there are still few species cataloged and strictly studied as their therapeutic potential, which makes it difficult to make use of these. However, ethnobotany research in the Northeast has shown satisfactory results, especially of plants adapted in the caatinga [5]. The caatinga biome is present only in The Brazilian territory, with an area of 844,453 km2 , which corresponds to 11.00% of the national territory, predominant of all states of the Northeast and North region of Minas Gerais [6]. Thus, it is possible to emphasize the species belonging to the family Caparaceae more precisely the species Capparis flexuosa L.
(Caparaceae), which is popularly known as wild beans, stands out for presenting a favorable development in semiarid regions, such as the northeast, and in a dry period the occurrence by biological production is satisfactory of this species. Its morphological characteristics consist of a shrub-tree size of perennial leaves, in the late afternoon insects and bees become attracted by their flowers, because they have the ability to ezalarem odor, more precisely due to the main floral resource that it has nectar [10]. Due to its meliferous relevance, C. flexuosa L. is recommended to be cultivated in regions that have as activity the creation and conservation of bees, for the extraction of honey. In addition, this forage exercise an important activity in cattle ranching activities in the northeast, which serve as food for the herds, mainly for sheep and goats, thanks to their resistance to develop in dry periods [11].
In traditional medicine, wild beans are presented as a contributor to several diseases, such as: sinusitis through its inhalation, and against snake bite reactions, from the embehest of the liquid resulting from its peel shaved with water. In view of the attributions of wild beans in traditional medicine, it is important to quantify phenolic compounds and flavonoids of the leaves of C.
Therefore, the objective of this study was to quantify the total phenol and flavonoid content of the leaves of C. flexuosa L, using spectrophotometric techniques.

Obtaining the Crude Ethanol Extract
The leaves were collected, identified, dried at room temperature and subsequently crushed. The extraction of the chemical constituents was performed by macerated in P.A. ethanol for two weeks. The extractor liquid was concentrated in rotaevaporator at a temperature of 45 °C degrees Celsius, to obtain the crude extract.

Phenolic Compounds -Folin Ciocalteau
The quantification of phenolic compounds and flavonoids was performed by the analytical method of quantification by external standardization, with the aid of a UV-VIS spectrophotometer. The method for determination of total phenols was adapted from [16],

Quantification of Flavonoid Content
The analysis was adapted to the study by [17]. Initially, the calibration curve was prepared with the quercetin pattern, where